MABT833

Sigma-Aldrich

Anti-Galectin-9 Antibody, clone 1G3

clone 1G3, from mouse

Synonyme(s):
Galectin-9, Gal-9, Ecalectin, Tumor antigen HOM-HD-21, Galectin-9
eCl@ss:
32160702
NACRES:
NA.41

Niveau de qualité

100

Source biologique

mouse

antibody product type

primary antibodies

Clone

1G3, monoclonal

species reactivity

human

Application(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

Isotype

IgG1κ

N° d'accession NCBI

N° d'accession UniProt

Expédié(e)(s) dans

wet ice

Gene Information

human ... LGALS9(3965)

Description générale

Galectin-9 (UniProt O00182; also known as Ecalectin, Gal-9, Tumor antigen HOM-HD-21, Urate transporter/channel protein) is encoded by the LGALS9 (also known as HUAT, LGALS9A) gene (Gene ID 3965) in human. The beta-galactoside-binding lectin, Galectin-9, possesses two distinct carbohydrate recognition domains (CRDs) linked together by a peptide domain of different length among the S, M, and L spliced isoforms. Galectin-9 plays a key role in a negative feed-back mechanism against Th1 immune response, where galectin-9 production from various cell types (e.g. fibroblasts and endothelial cells) is induced by interferon-gamma produced by CD4+ Th1 lymphocytes. The up-regulated galectin-9 in turn suppresses CD4+ Th1 lymphocytes, at least in part through stimulation of the Tim-3 receptor. The Tim-3 receptor on CD4+ Th1 cells from patients with multiple sclerosis (MS), rheumatoid arthritis, and auto-immune hepatitis is defective in its response to galectin-9. In addition, excessive galectin-9 production is reported in two human diseases associated with oncogenic viruses, nasopharyngeal carcinomas (NPC) associated with the Epstein-Barr virus (EBV) and chronic infection by the hepatitis C virus (HCV).

Spécificité

Clone 1G3 reacts with all three (L, M, and S) galectin-9 isoforms, but not galectin 1, 2, 3, 4, 7, 8, or 10.

Immunogène

GST-tagged recombinant protein corresponding to human Galectin-9.

Application

This Anti-Galectin-9 Antibody, clone 1G3 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), ELISA and Immunocytochemistry for the detection of Galectin-9.
Immunohistochemistry Analysis: A representative lot detected galectin-9 immunoreactivity in hepatocytes as well as inflammatory leucocytes and Kupffer cells in paraffin-embedded liver sections from patients with hepatitis C or B infection, but not in non-infected liver specimens (Barjon, C., et al. (2012). Infect Agent Cancer. 7(1):16-26).
Immunohistochemistry Analysis: A representative lot detected galectin-9 immunoreactivity in malignant cells using various paraffin-embedded nasopharangeal carcinoma (NPC) tissue sections (Barjon, C., et al. (2012). Infect Agent Cancer. 7(1):16-26).
Western Blotting Analysis: A representative lot detected endogenous galectin-9 isoforms in HeLa, lymphoblastoid cell line (LCL) REMB1, and nasopharyngeal carcinoma (NPC) cell lines C15 & C666-1, as well as exogenously expressed galectin-9 isoforms in transfected HeLa cells, but not in Burkitt’s lymphoma (BL) BL2 cells (Barjon, C., et al. (2012). Infect Agent Cancer. 7(1):16-26).
ELISA Analysis: A representative lot selectively captured human & murine galectin-9 (mGal-9 M, hGAL-9 S & M isoforms), but not human galectin 1, 2, 3, 4, 7, 8, or 10 (Barjon, C., et al. (2012). Infect Agent Cancer. 7(1):16-26).
Immunocytochemistry Analysis: A representative lot detected galectin-9 immunoreactivity in formalin-fixed and paraffin-embedded lymphoblastoid cell line (LCL) REMB1, but not in Burkitt’s lymphoma (BL) BL2 cells (Barjon, C., et al. (2012). Infect Agent Cancer. 7(1):16-26).

Qualité

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Galectin-9 in 10 µg of HeLa nuclear extract.

Description de la cible

~38 kDa observed

Forme physique

Format: Purified

Autres remarques

Concentration: Please refer to lot specific datasheet.

WGK Allemagne

WGK 1

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

A novel monoclonal antibody for detection of galectin-9 in tissue sections: application to human tissues infected by oncogenic viruses.
Barjon, C; Niki, T; Verillaud, B; Opolon, P; Bedossa, P; Hirashima, M; Blanchin et al.
Infectious Agents and Cancer null

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique

Réseaux sociaux

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

Merck

Recherche. Développement. Production

Nous sommes un des tout premiers fournisseurs du secteur mondial des Sciences de la vie proposant des solutions et des services pour la recherche, le développement et la production en biotechnologies, et le développement et la production de produits thérapeutiques pharmaceutiques.

© 2021 Merck KGaA, Darmstadt, Allemagne et/ou ses sociétés affiliées. Tous droits réservés.

La reproduction d'une quelconque partie du contenu de ce site est strictement interdite sans autorisation.