Merck
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10843555001

Roche

Hygromycin B

from Streptomyces hygroscopicus

Synonym(s):
Hygromycin B from Streptomyces hygroscopicus
Empirical Formula (Hill Notation):
C20H37N3O13
CAS Number:
Molecular Weight:
527.52
Numéro MDL:
ID de substance PubChem:

Source biologique

Streptomyces hygroscopicus

Stérilité

non-sterile; 0.2 μm filtered

Essai/Dosage

80% (HPLC)

Forme

buffered aqueous solution

Composition

Hygromycin B, >80% HPLC

Conditionnement

pkg of 20 mL (1 g)

manufacturer/tradename

Roche

Concentration

50 mg/mL

technique(s)

transfection: suitable

Expédié(e)(s) dans

wet ice

SMILES string

CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O

InChI

1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1

InChI key

GRRNUXAQVGOGFE-XKIAHZFYSA-N

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Description générale

  • Formula: C20H37N3O13
  • LD50: 6 mg/kg in mouse (i.v.);13 mg/kg in guinea pig (i.p.); 63 mg/kg in rat (i.p.)
  • Molecular weight: Mr = 527.5
  • Working concentration: 50 - 1,000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used.

Application

Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. It is used to select and maintain the phenotype of eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene (hyg or hph).

Actions biochimiques/physiologiques

Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.

Qualité

Purity: >80% (HPLC)

Définition de l'unité

Unit Assay: Unit/mg
We do not have this information.

Forme physique

Solution, 50 mg/ml in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membrane

Notes préparatoires

Working concentration: Recommended concentration for the selection of resistant cells is 50 to 1000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be determined experimentally, and may vary with the type of cell used.

Stockage et stabilité

Store at 2 to 8 °C. (solution)

Autres remarques

For life science research only. Not for use in diagnostic procedures.
Solution, 50 mg/ml, in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membrane.

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 1 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Eye Dam. 1 - Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 1

Flash Point(F)

does not flash

Point d'éclair C

does not flash

Certificate of Analysis

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Certificate of Origin

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