Merck
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11681451001

Roche

NBT/BCIP Stock Solution

Synonym(s):
5-bromo-4-chloro-3-indoyl phosphate, BCIP

Forme

solution

Niveau de qualité

100

Poids mol.

817.7

Conditionnement

pkg of 8 mL

manufacturer/tradename

Roche

Temp. de stockage

2-8°C

Description générale

The reaction product has a blue color and is insoluble in water. The blue color can be visually seen.

Formulas: NBT: C40H30Cl2N10O6, BCIP: C8H6NO4BrClP x C7H9N

Evaluation: visually

Application

  • The solution is used for the sensitive detection of alkaline phosphatase (AP) in blotting protocols, including: Southern blot
  • Northern blot
  • Western blot
  • Colony and plaque lifts
  • In situ hybridization
  • and for applications in immunohistochemistry and immunocytochemistry.
Note: NBT/BCIP may be used with both nitrocellulose and nylon membranes.

Forme physique

Solution of 18.75 mg/ml nitro blue tetrazolium chloride and 9.4 mg/ml 5-bromo-4-chloro-3-indolyl-phosphate, toluidine-salt in 67% (DMSO) (v/v).
Note: The color of the solution can vary from yellowish to light brown.

Notes préparatoires

Working solution: Preparation of staining solution
Always prepare this solution fresh shortly before use.
  • For all applications except DIG System: Add 200 μl of the stock solution to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl, 0.05 M MgCl2.
  • For DIG System applications: Add 200 μl of the stock solution to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl
Note: Do not include MgCl2 in the detection buffer as this may lead to spotty background on the membrane after the detection procedure. Insoluble in water.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Point d'éclair C

does not flash

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