TAQHSKB

Roche

KAPA Taq HotStart

NACRES:
NA.55

Niveau de qualité

100

Durée de conservation

≤18 mo.

Conditionnement

pkg of 250 U (KK1508)
pkg of 2500 U (KK1513)
pkg of 500 U (KK1510)

manufacturer/tradename

Roche

Expédié(e)(s) dans

dry ice

Temp. de stockage

−20°C

Vous recherchez des produits similaires ? Visit Guide de comparaison des produits

Application

KAPA Taq HotStart® has been used for:
  • High throughput PCR
  • Amplification of low copy DNA templates
  • Multiplex PCR
  • Specific amplification of complex templates
  • RT-PCR
  • confronting two-pair primers-polymerase chain reaction (CTPP-PCR)
  • end-point PCR

Actions biochimiques/physiologiques

KAPA Taq DNA Polymerase is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart® DNA Polymerase have 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′ → 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. In the HotStart formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.

Caractéristiques et avantages

High performance
  • Improved sensitivity, specificity, and yields.
  • Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific product.

Quick Notes:
  • KAPA Taq HotStart® DNA Polymerase can replace any commercial hotstart Taq DNA polymerase in an existing protocol. The final MgCl2 concentration and annealing temperature may need to be optimized to account for differences in formulation.
  • The KAPA Taq HotStart Buffer is a uniquelyformulated buffer offering improved specificity and sensitivity, and improved amplification of GC- and AT-rich templates.
  • The KAPA Taq HotStart Buffer does not contain MgCl2; MgCl2 (25 mM) is supplied separately to allow greater flexibility during reaction setup.
  • The KAPA Taq HotStart PCR Kit is suitable for the amplification of fragments up to 3.5 kb from genomic DNA or 5 kb from less complex targets.

Qualité

Each batch of KAPA Taq HotStart® DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA Taq HotStart PCR kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.

Notes préparatoires

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long term storage.

Autres remarques

For Research Use Only. Not for use in diagnostic procedures.

Informations légales

HOTSTART is a registered trademark of Molecular BioProducts, Inc.

Composants de kit seuls

Réf. du produit
Description

  • KAPA Taq Standard or HotStart® DNA Polymerase 5 U/μL

  • 10X KAPA Taq Buffer A

  • 10X KAPA Taq Buffer B

  • 10X KAPA Buffer with loading dye (optional)

  • 5X KAPA Taq HotStart® Buffer (HotStart kits only)

  • MgCl2 25 mM

Pictograms

Exclamation markHealth hazard

Mention d'avertissement

Warning

Mentions de danger

RID ADR

NONH for all modes of transport

WGK Allemagne

WGK 1

Flash Point(F)

does not flash

Point d'éclair C

does not flash

Certificat d'analyse
Certificat d'origine
Functional characterisation of a Fads2 fatty acyl desaturase with Δ6/Δ8 activity and an Elovl5 with C16, C18 and C20 elongase activity in the anadromous teleost meagre (Argyrosomus regius).
Monroig O, et al.
Aquaculture (Amsterdam, Netherlands), 412, 14-22 (2013)
The VNTR 48 bp polymorphism in the DRD4 gene is associated with higher tobacco smoking in male Mexican mestizo smokers with and without COPD
Perez-Rubio G, et al.
Diagnostics (Basel, Switzerland), 10 (2020)
Confronting two biomolecular techniques to detect NRF2 gene polymorphism biomarkers
Chiarella P, et al.
Future science OA, 5 (2018)
Articles
An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.
Lire la suite

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique

Réseaux sociaux

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

Merck

Recherche. Développement. Production

Nous sommes un des tout premiers fournisseurs du secteur mondial des Sciences de la vie proposant des solutions et des services pour la recherche, le développement et la production en biotechnologies, et le développement et la production de produits thérapeutiques pharmaceutiques.

© 2021 Merck KGaA, Darmstadt, Allemagne et/ou ses sociétés affiliées. Tous droits réservés.

La reproduction d'une quelconque partie du contenu de ce site est strictement interdite sans autorisation.