The product is not tested for RNase or DNase. Please see the link below to review Triton X-100 product options that include RNase and DNase testing:
https://www.sigmaaldrich.com/product/compare?term=triton%20x-100%20nuclease&focus=products&page=1&perpage=30&sort=relevance&type=product&products=T8787%3ASIGMA%3Asial%2C648466%3AMM%3Asial&returnUrl=%2Fsearch%2Ftriton-x-100-nuclease
X100
Triton™ X-100
Triton™ X-100
laboratory grade
Synonyme(s) :
t-Octylphénoxypolyéthoxyéthanol, Éther tert-octylphénylique du polyéthylène glycol
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Sélectionner une taille de conditionnement
5 ML
39,10 €
100 ML
71,10 €
500 ML
87,30 €
1 L
120,00 €
1 GAL
373,00 €
5 GAL
1 470,00 €
Sélectionner une taille de conditionnement
Changer de vue
5 ML
39,10 €
100 ML
71,10 €
500 ML
87,30 €
1 L
120,00 €
1 GAL
373,00 €
5 GAL
1 470,00 €
About This Item
Formule linéaire :
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
Numéro CAS:
Beilstein:
2315025
Numéro MDL:
Code UNSPSC :
12161900
ID de substance PubChem :
Nomenclature NACRES :
NA.21
Produits recommandés
Source biologique
synthetic
Niveau de qualité
Qualité
laboratory grade
Description
non-ionic
Forme
liquid
Poids mol.
micellar avg mol wt 80,000
average mol wt 625
Nombre d'agrégation
100-155
CMC
0.2-0.9 mM (20-25°C)
Pf
6 °C (43 °F)
Température de transition
cloud point 65 °C
pour point ~7 °C
Solubilité
water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow
HLB
13.5
Chaîne SMILES
CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
InChI
1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
Clé InChI
HNLXNOZHXNSSPN-UHFFFAOYSA-N
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Catégories apparentées
Description générale
Le Triton™ X-100 est un tensioactif non ionique courant et un émulsifiant fréquemment utilisé pour la solubilisation des protéines dans les applications biochimiques. Ce détergent non dénaturant est considéré comme relativement doux, et son utilisation en tant que réactif de routine a été mentionnée dans de nombreux articles. Il est utilisé pour lyser les cellules et en extraire les protéines et organites cellulaires. Il peut également perméabiliser les membranes de cellules vivantes pour la transfection.[1][2]
Application
Le Triton™-X a été utilisé :
- pour la perméabilisation cellulaire lors de marquage par immunofluorescence[5]
- en tant que composant de tampon de lyse lors d'analyse par western blotting[5]
- en tant que composant de tampon Tris salin lors de la préparation de coupes cellulaires pour la technique d'immunomarquage à l'or en microscopie électronique[5]
Actions biochimiques/physiologiques
Ce produit est couramment utilisé comme surfactant non ionique pour la récupération des composants membranaires dans des conditions douces non dénaturantes.
Caractéristiques et avantages
- Produit idéal pour les applications de recherche en biologie cellulaire et en biochimie.
- Détergent non ionique non dénaturant.
- Agent mouillant et émulsifiant performant.
- Tensioactif extrêmement polyvalent.
Autres remarques
Pour de plus amples informations sur notre gamme de Produits biochimiques, merci de compléter ce formulaire.
Informations légales
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
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Description
Tarif
Souvent commandé avec ce produit
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
483.8 °F - closed cup
Point d'éclair (°C)
251 °C - closed cup
Équipement de protection individuelle
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter, type N95 (US)
Listes réglementaires
Les listes réglementaires sont principalement fournies pour les produits chimiques. Seules des informations limitées peuvent être fournies ici pour les produits non chimiques. L'absence d'indication signifie qu'aucun des composants n'est répertorié. Il incombe à l'utilisateur de s'assurer de l'utilisation sûre et légale du produit.
EU REACH SVHC Candidate List
CAS No.
EU REACH Annex XIV (Authorisation List)
CAS No.
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Certificats d'analyse (COA)
Lot/Batch Number
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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
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Articles
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Protocoles
Enzymatic activity assay for cholesterol oxidase, excluding specific product numbers, providing guidelines for accurate cholesterol oxidase assays.
Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.
This page shows how to solubilize membrane proteins with products from Cytiva.
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Is this product nuclease-free?
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How long is Triton X-100 stable at -80 degrees in buffer?
1 réponse-
At -80°C, Triton X-100 is generally stable, but the stability can depend on the specific buffer conditions and the concentration of the detergent. In most cases, Triton X-100 remains effective at low temperatures, but it is advisable to avoid repeated freeze-thaw cycles, as these can lead to changes in the detergent's properties and effectiveness. Please note that the shelf life after opening is dependent upon various factors which might affect the stability and, therefore, it is not guaranteed and needs to be determined by the end user.
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If a sterilization of Triton X-100 or its dilutions (maximum 10% of Triton X-100 in the total volume) is needed to remove DNAse and RNAse, what's the best approach?
1 réponse-
Autoclaving undiluted Triton X-100 is generally not recommended for sterilization. However, dilute solutions, up to 10%, may be autoclaved. At certain
concentrations, solutions may become cloudy, but dispersible above the cloud point. The solution will clarify with stirring upon cooling. Note that DNAse and RNAse-free products are currently available. Please see the links below to review these product options:
https://www.sigmaaldrich.com/product/sigma/t8787
https://www.sigmaaldrich.com/product/mm/648466
https://www.sigmaaldrich.com/product/sigma/93443Utile ?
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfUtile ?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 réponse-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What are the storage conditions for the Triton 100x? It's not in the COA
1 réponse-
This product may be be stored in room temperature.
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Will heating Triton to reduce its viscosity have any negative impact on the product? Does heating negatively affect the activity of Triton?
1 réponse-
Triton X-100 can be frozen, thawed, and used. However, caution is advised when heating it above 50°C, as discoloration may occur. This note indicates that excessive heating can lead to some form of change in Triton X-100. It would be reasonable to assume that the recommended working range is from ambient temperature to 50°C (or slightly lower). If your process requires heating the solution to temperatures higher than 50°C, it is recommended to add Triton X-100 only after the temperature has dropped below 50°C or lower. If you need to work at temperatures exceeding 50°C, it is advisable to conduct solution stability studies to assess potential damage or breakdown of Triton X-100.
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Is a concentration of 8x10^-5 mol/L of Triton X-100 harmful to metal (steel/aluminum) and silicon equipment when submerged in water?
1 réponse-
The laboratory has not specifically tested the corrosive properties of Triton X-100 on metal. However, according to a publication titled "Triton X-100 and Sodium Lauryl Sulfate Surfactants as Environmentally Friendly Corrosion Inhibitors for Steel in 0.5 M H2SO4" in Advances in Analytical Chemistry, Triton X-100 appears to act as a corrosion inhibitor for steel.
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What is the correct CAS number for Triton X-100, 9002-93-1 or 9036-19-5?
1 réponse-
CAS 9036-19-5 is a generic CAS number and is correct for Triton® X-100. With this number, neither the substitution pattern of the aromatic ring (para, meta, ortho), nor the amount of the repeating units of the oxyethylene units is specified or described. Triton® X-100 with CAS 9002-93-1 is also correct but it defines only the para-substitution.
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What are the NFPA hazard numbers for this?
1 réponse-
With the implementation of the Globally Harmonized System (GHS) of hazard classification in our safety data sheets (SDS), we decided to no longer maintain the National Fire Protection Association (NFPA) information in section 15 of the SDS. Therefore, we no longer provide updates to the NFPA ratings. Kindly review the SDS available at this link: https://www.sigmaaldrich.com/US/en/sds/sial/x100?userType=anonymous
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