This product is not a certified reference material, nor has it been tested for use in HPLC/MS. As Triton X-100 is typically avoided in Mass Spectrometry, there is no analytical standard currently that is qualified for this application. The end user would have to determine suitability for use in HPLC/MS. However, this material is referenced in an LC/MS publication by Shimadzu. Please see the link below to review:
https://www.shimadzu.com/an/sites/shimadzu.com.an/files/pim/pim_document_file/technical/white_papers/13844/apo21759.pdf
X100
Triton™ X-100
laboratory grade
Triton™ X-100
Synonyme(s) :
t-Octylphénoxypolyéthoxyéthanol, Éther tert-octylphénylique du polyéthylène glycol
Se connecter pour consulter les tarifs organisationnels et contractuels.
Sélectionner une taille de conditionnement
Changer de vue
| Conditionnement | Référence | Disponibilité | Prix |
|---|---|---|---|
| 5 mL | Expédition prévue aujourd'huiDeSt QuentinFallavier | 41,10 € | |
| 100 mL | Expédition prévue aujourd'huiDeSt QuentinFallavier | 74,30 € | |
| 500 mL | Expédition prévue aujourd'huiDeSt QuentinFallavier | 91,30 € | |
| 1 L | Expédition prévue aujourd'huiDeSt QuentinFallavier | 125,00 € | |
| 1 gal | Vérifier la disponibilité des articles du panier | 392,00 € | |
| 5 gal | Vérifier la disponibilité des articles du panier | 1 520,00 € |
A propos de cet article
Formule linéaire :
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
Numéro CAS:
UNSPSC Code:
12161900
NACRES:
NA.21
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
2315025
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderbiological source
synthetic
Quality Level
grade
laboratory grade
description
non-ionic
form
liquid
mol wt
micellar avg mol wt 80,000, average mol wt 625
aggregation number
100-155
CMC
0.2-0.9 mM (20-25°C)
mp
6 °C (43 °F)
transition temp
cloud point 65 °C, pour point ~7 °C
solubility
water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow
HLB
13.5
application(s)
advanced drug delivery
SMILES string
CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
InChI
1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
InChI key
HNLXNOZHXNSSPN-UHFFFAOYSA-N
General description
Le Triton™ X-100 est un tensioactif non ionique courant et un émulsifiant fréquemment utilisé pour la solubilisation des protéines dans les applications biochimiques. Ce détergent non dénaturant est considéré comme relativement doux, et son utilisation en tant que réactif de routine a été mentionnée dans de nombreux articles. Il est utilisé pour lyser les cellules et en extraire les protéines et organites cellulaires. Il peut également perméabiliser les membranes de cellules vivantes pour la transfection.[1]
Le Triton X-100 a des propriétés hémolytiques et est utilisé dans l′extraction d′ADN.
Application
Le Triton™-X a été utilisé :
- pour la perméabilisation cellulaire lors de marquage par immunofluorescence[2]
- en tant que composant de tampon de lyse lors d'analyse par western blotting[2]
- en tant que composant de tampon Tris salin lors de la préparation de coupes cellulaires pour la technique d'immunomarquage à l'or en microscopie électronique[2]
Biochem/physiol Actions
Ce produit est couramment utilisé comme surfactant non ionique pour la récupération des composants membranaires dans des conditions douces non dénaturantes.
Features and Benefits
- Produit idéal pour les applications de recherche en biologie cellulaire et en biochimie.
- Détergent non ionique non dénaturant.
- Agent mouillant et émulsifiant performant.
- Tensioactif extrêmement polyvalent.
Other Notes
Pour de plus amples informations sur notre gamme de Produits biochimiques, merci de compléter ce formulaire.
Legal Information
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
1 of 1
Cet article | |||
|---|---|---|---|
| grade laboratory grade | grade laboratory grade | grade - | grade Molecular Biology |
| form liquid | form - | form liquid | form liquid |
| aggregation number 100-155 | aggregation number - | aggregation number 100-155 | aggregation number 100-155 |
| application(s) advanced drug delivery | application(s) - | application(s) advanced drug delivery | application(s) hematology |
| mp 6 °C (43 °F) | mp - | mp - | mp 6 °C |
| solubility water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow | solubility - | solubility water: soluble | solubility water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow |
Still not finding the right product?
Explore all of our products under Triton™ X-100
signalword
Danger
Classe de stockage
10 - Combustible liquids
flash_point_f
483.8 °F - closed cup
flash_point_c
251 °C - closed cup
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter, type N95 (US)
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2
Listes réglementaires
Les listes réglementaires sont principalement fournies pour les produits chimiques. Seules des informations limitées peuvent être fournies ici pour les produits non chimiques. L'absence d'indication signifie qu'aucun des composants n'est répertorié. Il incombe à l'utilisateur de s'assurer de l'utilisation sûre et légale du produit.
9036-19-5
CAS No.
9036-19-5
CAS No.
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Protocoles
Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.
This page shows how to solubilize membrane proteins with products from Cytiva.
Enzymatic activity assay for cholesterol oxidase, excluding specific product numbers, providing guidelines for accurate cholesterol oxidase assays.
Articles
Millicell® hanging cell culture inserts for staining and microscopic analysis directly from the insert. Learn to utilize hanging cell culture inserts in barrier measurements.
Growth and hydration of Triton X-100 micelles in monovalent alkali salts: a light scattering study
Molina-Bolivar JA, et al.
The Journal of Physical Chemistry B, 106(4), 870-877 (2002)
Knockdown of miR-210 decreases hypoxic glioma stem cells stemness and radioresistance
Yang W, et al.
Experimental Cell Research, 326(1), 22-35 (2003)
José Francisco Lima-Barbero et al.
Experimental & applied acarology, 78(4), 555-564 (2019-08-02)
Blood-feeding ectoparasites constitute a growing burden for human and animal health, and animal production worldwide. In particular, mites (Acari: Gamasida) of the genera Dermanyssus (Dermanyssidae) and Ornithonyssus (Macronyssidae) infest birds and cause gamasoidosis in humans. The tropical fowl mite, Ornithonyssus
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| 82014-U | 04061838551214 |
| 82028-U | 04061833408612 |
| 82011-U | 04061838551191 |
| 82017-U | 04061833408643 |
| 82026-U | 04061833520598 |
| 82020-U | 04061833520697 |
| 82023-U | 04061833520581 |
| 82035-U | 04061833520659 |
| 82039-U | 04061833508633 |
| 82042-U | 04061833508657 |
| X100-100ML | 04061837603044 |
| X100-1L | 04061837603068 |
| X100-2ML | 04061837603075 |
| X100-5GA | 04061837603099 |
| X100-6X500ML | 04061837603112 |
| X100-500ML | 04061837603082 |
| X100-5ML | 04061837603105 |
| X100-1GA | 04061837603051 |
| HAM79433-1EA | 04061838551757 |
-
Salve Questo è uno standard Certificato oppure un materiale di riferimento? Posso usarlo come standard per retta di taratura in HPLC_MS?
1 réponse-
Utile ?
-
-
How can I determine the shelf life / expiration / retest date of this product?
1 réponse-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfUtile ?
-
-
What kind of change will be observed if heat up to 55C? Will it attack stacked Al and Ti metal layer? Can it mix with diluted nitric acid to etch Cu/NiV?
1 réponse-
Heating this product to 55°C may lead to changes in viscosity, solubility, or performance characteristics. There is no indication that this product can attack aluminum and titanium metal layers. This product is generally compatible with diluted nitric acid. While no specific incompatibility is reported, the combination may alter the properties of both the surfactant and the acid, potentially affecting the performance of either in applications such as cleaning or etching. Additionally, it is important to consider the concentration and the potential for foaming or other reactions during mixing.
Utile ?
-
-
Is this product nuclease-free?
1 réponse-
The product is not tested for RNase or DNase. Please see the link below to review Triton X-100 product options that include RNase and DNase testing:
https://www.sigmaaldrich.com/product/compare?term=triton%20x-100%20nuclease&focus=products&page=1&perpage=30&sort=relevance&type=product&products=T8787%3ASIGMA%3Asial%2C648466%3AMM%3Asial&returnUrl=%2Fsearch%2Ftriton-x-100-nucleaseUtile ?
-
-
How long is Triton X-100 stable at -80 degrees in buffer?
1 réponse-
At -80°C, Triton X-100 is generally stable, but the stability can depend on the specific buffer conditions and the concentration of the detergent. In most cases, Triton X-100 remains effective at low temperatures, but it is advisable to avoid repeated freeze-thaw cycles, as these can lead to changes in the detergent's properties and effectiveness. Please note that the shelf life after opening is dependent upon various factors which might affect the stability and, therefore, it is not guaranteed and needs to be determined by the end user.
Utile ?
-
-
If a sterilization of Triton X-100 or its dilutions (maximum 10% of Triton X-100 in the total volume) is needed to remove DNAse and RNAse, what's the best approach?
1 réponse-
Autoclaving undiluted Triton X-100 is generally not recommended for sterilization. However, dilute solutions, up to 10%, may be autoclaved. At certain
concentrations, solutions may become cloudy, but dispersible above the cloud point. The solution will clarify with stirring upon cooling. Note that DNAse and RNAse-free products are currently available. Please see the links below to review these product options:
https://www.sigmaaldrich.com/product/sigma/t8787
https://www.sigmaaldrich.com/product/mm/648466
https://www.sigmaaldrich.com/product/sigma/93443Utile ?
-
-
How is shipping temperature determined? And how is it related to the product storage temperature?
1 réponse-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Utile ?
-
-
What are the storage conditions for the Triton 100x? It's not in the COA
1 réponse-
This product may be be stored in room temperature.
Utile ?
-
-
Is a concentration of 8x10^-5 mol/L of Triton X-100 harmful to metal (steel/aluminum) and silicon equipment when submerged in water?
1 réponse-
The laboratory has not specifically tested the corrosive properties of Triton X-100 on metal. However, according to a publication titled "Triton X-100 and Sodium Lauryl Sulfate Surfactants as Environmentally Friendly Corrosion Inhibitors for Steel in 0.5 M H2SO4" in Advances in Analytical Chemistry, Triton X-100 appears to act as a corrosion inhibitor for steel.
Utile ?
-
-
Will heating Triton to reduce its viscosity have any negative impact on the product? Does heating negatively affect the activity of Triton?
1 réponse-
Triton X-100 can be frozen, thawed, and used. However, caution is advised when heating it above 50°C, as discoloration may occur. This note indicates that excessive heating can lead to some form of change in Triton X-100. It would be reasonable to assume that the recommended working range is from ambient temperature to 50°C (or slightly lower). If your process requires heating the solution to temperatures higher than 50°C, it is recommended to add Triton X-100 only after the temperature has dropped below 50°C or lower. If you need to work at temperatures exceeding 50°C, it is advisable to conduct solution stability studies to assess potential damage or breakdown of Triton X-100.
Utile ?
-