32670

4′,6-Diamidino-2-phenylindole dihydrochloride

BioReagent, suitable for fluorescence, ≥95.0% (HPLC)

• Synonym(s): 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride
• Empirical Formula (Hill Notation): C₁₆H₁₅N₅ · 2HCl
• CAS Number: 28718-90-3
• Molecular Weight: 350.25
• Beilstein: 4894417
• Numéro EC: 249-186-7
• Numéro MDL: MFCD00012681
• ID de substance PubChem: 57648699
• NACRES: NA.32

Properties

• Gamme de produits: BioReagent
• Niveau de qualité: 100
• Essai/Dosage: ≥95.0% (HPLC)
• Forme: powder
• Solubilité: DMF: soluble; H₂O: soluble
• Fluorescence: λ_ex 340 nm; λ_em 488 nm (nur DAPI); λ_ex 364 nm; λ_em 454 nm (DAPI-DNA-Komplex); λ_ex 374 nm; λ_em 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA
• Adéquation: suitable for fluorescence
• SMILES string: Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
• InChI: 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
• InChI key: FPNZBYLXNYPRLR-UHFFFAOYSA-N

Description

Application

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Conditionnement

5, 25 mg

Bottomless glass bottle. Contents are inside inserted fused cone.

Actions biochimiques/physiologiques

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Safety Information

• Pictograms: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H315 - H317 - H335
• Conseils de prudence: P261 - P264 - P271 - P272 - P280 - P302 + P352
• Classification des risques: Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Point d'éclair C: Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)