66540305

Sigma-Aldrich

WTSIi151-A

Human iPS Cell Line

Synonyme(s):
EBiSC iPSC Line, iPSC, Human iPSC, iPS Cell, Induced Pluripotent Stem Cell Line

Source biologique

human dermis (fibroblast)

Méthode de reprogrammation

Sendai virus

Description

age (25-29)

manufacturer/tradename

EBiSC

Sexe

male

Mode de croissance

adherent (pluripotent)

Application(s)

cell culture | stem cell: suitable

Maladie(s) pertinente(s)

Bardet-Biedl syndrome

Expédié(e)(s) dans

dry ice

Temp. de stockage

−196°C

Catégories apparentées

Description générale

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The
EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Origine de la lignée cellulaire

Depositor
Wellcome Trust Sanger Institute

Description de la lignée cellulaire

Derivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Arm
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown

Characterization
Analysis of Undifferentiated Cells
Marker expression: NANOG(+)POU5F1 (OCT-4)(+)SOX2(+)
PluriTest: Pluripotency score: 34.624
Novelty score: 1.452

Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative

Genotyping
Genome-wide analysis:
Exome sequencing
https://www.ebi.ac.uk/ega/studies/EGAS00001000969

Liaison

Note: EAUA and CLIP must be completed before order fulfillment

Procédure de repiquage

Surface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
CO2 concentration: 5%
Culture medium: TeSR® E8®

Informations légales

E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
TeSR is a registered trademark of WiCell Research Institute, Inc.

RID ADR

NONH for all modes of transport

WGK Allemagne

WGK 3

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

Certificat d'analyse
Certificat d'origine

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