66540606

Sigma-Aldrich

BIONi037-A-4

Human iPS Cell Line

Source biologique

human

Méthode de reprogrammation

episomal

Description

age (N/A)

manufacturer/tradename

EBiSC

Sexe

female

Mode de croissance

adherent (pluripotent)

Application(s)

cell culture | stem cell: suitable

Maladie(s) pertinente(s)

Alzheimer′s disease

Expédié(e)(s) dans

dry ice

Temp. de stockage

−196°C

Catégories apparentées

Description générale

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The
EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Origine de la lignée cellulaire

Depositor
Bioneer

Description de la lignée cellulaire

Derivation
Primary cell type: -
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr

Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no

Characterization
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative

Genotyping

Karyotyping
Cell line karyotype: 46 XY
Karyotyping method: G-Banding
Image / Result

Genetic Modification
Disease/phenotype related modifications
Disease: Alzheimers disease
Type of modification: Isogenic
Gene: Apolipoprotein E
Chromosome location: 19q13.32
Nucleotide sequence HGSV: rs7412 (C/C); rs429358 (C/C)
Zygosity: Homozygous
Target locus modification: The bases at both SNPs rs7412 and rs429358 were changed to a C

Liaison

Note: EAUA and CLIP must be completed before order fulfillment

Procédure de repiquage

Culture medium: Essential E8®
No. Vials banked (pre-EBiSC): 30

Informations légales

E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation

RID ADR

NONH for all modes of transport

WGK Allemagne

WGK 3

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

Certificat d'analyse
Certificat d'origine

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