66540671

Sigma-Aldrich

STBCi017-B

Human iPS Cell Line

Source biologique

human dermis (fibroblast)

Méthode de reprogrammation

Sendai virus

Description

age (N/A)

manufacturer/tradename

EBiSC

Sexe

male

Mode de croissance

adherent (pluripotent)

Application(s)

cell culture | stem cell: suitable

Maladie(s) pertinente(s)

neuropathy

Expédié(e)(s) dans

dry ice

Temp. de stockage

−196°C

Catégories apparentées

Description générale

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The
EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Origine de la lignée cellulaire

Depositor
StemBANCC

Description de la lignée cellulaire

Derivation
Primary cell type: Fibroblast
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Is the reprogramming vector detectable: Unknown

Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no

Characterization
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative

Genotyping
Karyotyping
Cell line karyotype: No abnormalities detected
Karyotyping method: Molecular karyotyping by SNP array

Liaison

Note: EAUA and CLIP must be completed before order fulfillment

Procédure de repiquage

Surface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 21%
CO2 concentration: 5%
Culture medium: mTeSR® 1

Informations légales

GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.

RID ADR

NONH for all modes of transport

WGK Allemagne

WGK 3

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

Certificat d'analyse
Certificat d'origine

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