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A2153

Sigma-Aldrich

Bovine Serum Albumin

lyophilized powder, ≥96% (agarose gel electrophoresis)

Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
CAS Number:
Numéro EC:
Numéro MDL:
NACRES:
NA.27

Source biologique

bovine

Niveau de qualité

Essai/Dosage

≥96% (agarose gel electrophoresis)

Forme

lyophilized powder

Poids mol.

~66 kDa

Purifié(e)(s) par

cold ethanol fractionation

Origine

USA origin

technique(s)

ELISA: suitable
blood typing: suitable
microbiological culture: suitable

Perte

≤6%

pH

6.5-7.5 (1% in 0.15 M sodium chloride)
7

Solubilité

water: soluble (40 mg/ml)

N° d'accession UniProt

Temp. de stockage

2-8°C

Gene Information

bovine ... ALB(280717)

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Application

Bovine serum albumin has been used-
  • along with BTS (Beltsville thawing solution), for the extension of sperms during sperm motion analysis
  • as a component of modified Krebs-Ringer buffer (dmKRBT)
  • in gel shift analysis

Conditionnement

5, 10, 50, 100, 500 g in poly bottle
1, 5, 10 kg

Actions biochimiques/physiologiques

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Notes préparatoires

Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Code de la classe de stockage

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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