Merck
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C8035

Sigma-Aldrich

Monoclonal Anti-Chondroitin Sulfate antibody produced in mouse

clone CS-56, ascites fluid

Synonyme(s):
Chondroitin Sulfate Antibody, Cs56 Antibody, Chondroitin Sulfate Antibody - Monoclonal Anti-Chondroitin Sulfate antibody produced in mouse
Numéro MDL:
NACRES:
NA.41

Niveau de qualité

200

Source biologique

mouse

Conjugué

unconjugated

Forme d'anticorps

ascites fluid

antibody product type

primary antibodies

Clone

CS-56, monoclonal

species reactivity

chicken, human, bovine, rabbit, pig, rat, mouse

technique(s)

indirect immunofluorescence: 1:200 using bovine mammary gland epithelial (BMGE) cells
microarray: suitable

Isotype

IgM

N° d'accession UniProt

Expédié(e)(s) dans

dry ice

Temp. de stockage

−20°C

Gene Information

human ... ACAN(176)
mouse ... Acan(11595)
rat ... Acan(58968)

Description générale

Chondroitin sulfate is a linear polysaccharide composed of alternating disaccharide glucuronic acid and N-acetylgalactosamine. It contains proteoglycan (PG) of the extracellular matrix (ECM). Monoclonal Anti-Chondroitin Sulfate (mouse IgM isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
This product can be found as purified product that was produced using cell culture hybridoma (SAB4200696)

Spécificité

The antibody has been reported to be specific for the glycosaminoglycan (GAG) portion of native chondroitin sulfate proteoglycan (CSPG). The antibody reacts specifically with chondroitin sulfate types A and C but not with type B (dermatan sulfate), and may be used for localization of chondroitin sulfate in tissues and cultured fibroblasts.

Immunogène

Ventral membranes of chicken gizzard fibroblasts

Application

Monoclonal Anti-Chondroitin Sulfate antibody produced in mouse was used:
  • in immunohistochemistry as primary antibody to study of spinal cords and brain cryosections.
  • in ELISA and western blot for purification of soluble forms of myelin-associated glycoprotein (MAG) and neural cell adhesion molecule (N-CAM).
  • in flow cytometry studies.
  • as primary antisera in a study of TGF-β regulation.
Monoclonal Anti-Chondroitin Sulfate can also be used to study chondroitin sulfate proteoglycan (CSPG) distribution and its relationships to specific cell-substrate contacts. It is also suitable for microarray and indirect immunofluorescence at the dilution of 1:200 by using bovine mammary gland epithelial (BMGE) cells.

Actions biochimiques/physiologiques

Chondroitin sulfate is involved in tumor invasion and also plays a vital role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. In Chondroitin sulfate proteoglycan (CSPG), proteoglycan part is involved in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. Chondroitin sulfate is used in osteoarthritis treatment. Upregulation of chondroitin sulfate proteoglycans is observed after neuronal injury. It possess the ability to inhibit axonal regeneration.

Stockage et stabilité

For continuous use, store at 2-8 °C for up to one month.
For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Autres remarques

This product can be found as purified product that was produced using cell culture hybridoma product.
SAB4200696 Anti-Chondroitin Sulfate antibody, Mouse monoclonal
clone CS-56, purified from hybridoma cell culture

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

Certificat d'analyse

Saisir un numéro de lot pour rechercher un certificat d'analyse (COA).

Certificat d'origine

Saisir un numéro de lot pour rechercher un certificat d'origine (COO).

PTPσ is a receptor for chondroitin sulfate proteoglycan, an inhibitor of neural regeneration.
Shen Y, et al.
Science, 326(5952) (2009)
Charlotte Rolny et al.
The Journal of biological chemistry, 277(22), 19315-19321 (2002-03-26)
Platelet-derived growth factor (PDGF) induces mitogenic and migratory responses in a wide variety of cells, by activating specific receptor tyrosine kinases denoted the PDGF alpha- and beta-receptors. Different PDGF isoforms bind in a distinct manner to glycosaminoglycans, particularly heparan sulfate.
Effect of the counterion behavior on the frictional?compressive properties of chondroitin sulfate solutions
Polymer, 50(7), 1805-1813 (2009)
Z Avnur et al.
Cell, 38(3), 811-822 (1984-10-01)
Chondroitin-sulfate containing proteoglycan (CSPG) of the extracellular matrix (ECM) was visualized in chick tissues and cell cultures with a monoclonal antibody, CS-56. Cultured cells of various origins contained dense punctate layers of CSPG on both the substrate and the cell
R Probstmeier et al.
The Journal of cell biology, 116(4), 1063-1070 (1992-02-01)
To gain insights into the functional role of the molecular association between neural adhesion molecules and extracellular matrix constituents, soluble forms of the myelin-associated glycoprotein (MAG) and the neural cell adhesion molecule (N-CAM), representing most of the extracellular domains of

Articles

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

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