Merck
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CMC0014

Sigma-Aldrich

BL21(DE3) Chemically Competent Cells

for protein expression

NACRES:
NA.85

Qualité

for molecular biology

Transformation cellulaire

competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg

Expédié(e)(s) dans

dry ice

Temp. de stockage

−70°C

Description générale

The BL21(DE3) Chemically Competent strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Application

The E. coli strain BL21 (DE3) has been used to express tau 2N4R isoform (aa1–441) and the truncated tau (aa1–421) using pET19b as expression vector.

Caractéristiques et avantages

  • a transformation efficiency of >1 × 107 cfu/μg.
  • economical prices
  • offering superior value for everyday protein expression work.

Composants

  • BL21(DE3) chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression

Code de la classe de stockage

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

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Certificate of Origin

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