Merck
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CMC0016

Sigma-Aldrich

BL21(DE3) Electrocompetent Cells

for protein expression

Synonym(s):
BL21 strain
NACRES:
NA.85

Qualité

for molecular biology

Transformation cellulaire

competent cell type: electrocompetent
transformation efficiency: ≥5 × 109 cfu/μg

Expédié(e)(s) dans

dry ice

Temp. de stockage

−70°C

Description générale

The BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell.
Cloning efficiencies are increased 25-1,000 fold relative to other preparations of BL21 cells, which is essential for construction of complex expression libraries.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Caractéristiques et avantages

The unprecedented transformation efficiency of the BL21(DE3) Electrocompetent Cells (> 5 × 109 cfu/μg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment

Composants

  • BL21(DE3) electrocompetent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression


Code de la classe de stockage

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

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Certificate of Origin

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