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CMC0019

Sigma-Aldrich

OverExpress C43(DE3) Chemically Competent Cells

for the highest protein expression

Synonym(s):
C43 strain
NACRES:
NA.85

Qualité

for molecular biology

Transformation cellulaire

competent cell type: chemically competent
transformation efficiency: ≥1 x 106 cfu/μg

Expédié(e)(s) dans

dry ice

Temp. de stockage

−70°C

Description générale

OverExpress Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. The OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). OverExpress C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Application

OverExpress C43(DE3) Chemically Competent Cells have been used for transforming the toll/interleukin receptor (TIR) domain constructs of sterile alpha and TIR motif–containing protein 1 (SARM1).

Caractéristiques et avantages

  • Express genes cloned into any T7 vector with these BL21(DE3) derivatives
  • Effective in expressing toxic & membrane proteins
  • Cited in over 350 research articles

Composants

  • OverExpress C43(DE3) chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression


Informations légales

OverExpress is a trademark of Imaxio, S.A.

Code de la classe de stockage

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

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Certificate of Origin

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