CS0780

β-N-Acetylglucosaminidase Assay Kit

sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)

• Numéro EC: 222-398-7
• NACRES: NA.84

Properties

• Utilisation: sufficient for 50 reactions (1 mL); sufficient for 500 reactions (100 μL)
• Niveau de qualité: 200
• Expédié(e)(s) dans: wet ice
• Temp. de stockage: 2-8°C
• Gene Information: human ... NAGLU(4669)

Description

Application

The kit provides the reagents required for a fast and convenient detection of β-N-Acetylglucosaminidase activity in cell lysates, tissue homogenates or purified enzyme. It is a useful tool for the detection of lysosomes in fractionated cell/tissue samples.

Actions biochimiques/physiologiques

N-acetyl-D-glucosaminidase (NAG) in mammals is a lysosomal enzyme, which takes part in the intracellular degradation of glycolipids and glycoproteins. High activities of this enzyme have been detected in human kidney, lung and liver lysosomes. Elevated levels of serum NAG are associated with certain forms of cancer.

Remarque sur l'analyse

The kit assay is based on the hydrolysis of NAG substrate (NP-GLcNAc) by the enzyme. The enzymatic hydrolysis of the substrate liberates p-nitrophenylate ion. The reaction product is detected colorimetrically at 405 nm.

Kit Components Only

• Dilution Buffer 8 mL
• 4-Nitrophenyl-N-acetyl-β-D-glucosaminide 50 mg
• Citrate Buffer Solution, 0.09 M 100 mL
• p-Nitrophenol Standard Solution, 10 mM 1 mL
• β-N-Acetylglucosaminidase from Jack beans 1 vial
• Sodium carbonate 5 g

Safety Information

• Pictograms: GHS07,GHS08
• Mention d'avertissement: Warning
• Mentions de danger: H319 - H351 - H373
• Conseils de prudence: P202 - P260 - P264 - P280 - P305 + P351 + P338 - P308 + P313
• Classification des risques: Carc. 2 - Eye Irrit. 2 - STOT RE 2 Oral
• Code de la classe de stockage: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Point d'éclair C: Not applicable