This product is assigned an expiration date of approximately two years from the date of Quality Release. This date is lot specific and available on the product label and Certificate of Analysis. After opening, the material should be stored tightly sealed to prevent moisture uptake. Given proper handling and storage, the product should be stable until the published expiration date.
D2650
Diméthylsulfoxyde
Dimethyl sulfoxide
Hybri-Max™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Synonyme(s) :
DMSO, Sulfoxyde de méthyle
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About This Item
Formule linéaire :
(CH3)2SO
Numéro CAS:
Poids moléculaire :
78.13
Beilstein:
506008
Numéro CE :
Numéro MDL:
Code UNSPSC :
12191502
ID de substance PubChem :
Nomenclature NACRES :
NA.75
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Qualité
Hybri-Max™
Niveau de qualité
Densité de vapeur
2.7 (vs air)
Pression de vapeur
0.42 mmHg ( 20 °C)
Stérilité
sterile-filtered
Gamme de produits
BioReagent
Essai
≥99.7%
Forme
liquid
Température d'inflammation spontanée
573 °F
Limite d'explosivité
42 %, 63 °F
Technique(s)
cell culture | hybridoma: suitable
cryopreservation: suitable
Impuretés
endotoxin, tested
≤0.1% water
water
Indice de réfraction
n20/D 1.479 (lit.)
pb
189 °C (lit.)
Pf
16-19 °C (lit.)
Solubilité
H2O: miscible (completely)
Densité
1.10 g/mL (lit.)
Chaîne SMILES
CS(C)=O
InChI
1S/C2H6OS/c1-4(2)3/h1-2H3
Clé InChI
IAZDPXIOMUYVGZ-UHFFFAOYSA-N
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Application
Le DMSO est un solvant polaire aprotique, utilisé dans les réactions chimiques, dans les réactions en chaîne par polymérase (PCR) et comme agent de vitrification cryoprotecteur pour la préservation des cellules, des tissus et des organes. Le DMSO est utilisé dans les milieux de congélation de cellules pour les protéger des blessures mécaniques causées par les cristaux de glace. Il est utilisé pour la conservation par congélation de lignées cellulaires hétéroploïdes et d′hybridomes (primaires, issues de sous-cultures et recombinantes) ; de cellules souches embryonnaires (ESC) et de cellules souches hématopoïétiques. Le DMSO est fréquemment utilisé en combinaison avec l′albumine de sérum bovin (BSA) ou le sérum de veau fœtal (FBS).
Conditionnement
100 ml en flacon ambré
Le conditionnement de l'ampoule en verre est désormais disponible sous une nouvelle référence, D2653
Autres remarques
Ce produit est un produit Hybri-Max. Il a été testé avec des hybridomes et sa compatibilité avec la congélation de cellules a été évaluée. Ce produit a été stérilisé par filtration (0,2 µm) et ses taux d′endotoxines ont également été testés.
Attention
Refroidit facilement et revient doucement à la température de la pièce. Les produits solides peuvent être reliquéfiés par chauffage à la température ambiante sans abîmer le produit.
Informations légales
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
En option
Réf. du produit
Description
Tarif
Gants suggérés pour une protection contre les projections
Produit(s) apparenté(s)
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Description
Tarif
Souvent commandé avec ce produit
Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
188.6 °F - closed cup
Point d'éclair (°C)
87 °C - closed cup
Équipement de protection individuelle
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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Protocoles
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How many days after opening the bottle is DMSO safe to use?
1 réponse-
Utile ?
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I ordered 10 x 10ml vials for fibroblast culture work. My first endeavor in culture domain. What are the storage conditions for DMSO of this grade? This arrived in plain cardboard box at room temperature.
1 réponse-
DMSO is hygroscopic and light-sensitive. The storage container must protect from light and moisture uptake. Since this DMSO is sterile-filtered and intended for cell culture applications, the storage containers must also be sterile and endotoxin-free. It is recommended to purge aliquots with inert gas and store them in a compatible, tightly sealed container, at a cool storage area, such as 4°C. DMSO is not compatible with many types of plastics, so it is advisable to store it in appropriate amber glass vials for long-term storage. Please refer to the Safety Data Sheet and the Product Information Sheet for compatibility of DMSO with various materials and for further details.
Utile ?
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Is it safe in terms of health and product safety to store a leftover DMSO ampule covered with parafilm in a flammable cabinet?
1 réponse-
DMSO is hygroscopic and light-sensitive. The storage container must protect from light and moisture uptake. Since this DMSO is sterile-filtered and intended for cell culture applications, the storage containers must also be sterile and endotoxin-free. It is recommended to purge aliquots and store them under inert gas, in a compatible, tightly sealed container, at a cool storage area, such as 4°C.
DMSO is not compatible with many types of plastics, so it is advisable to store it in appropriate amber glass vials for long-term storage. Please refer to the Product Information Sheet and the Safety Data Sheet at the link below for compatibility of DMSO with various materials and for further details:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/944/265
Utile ?
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Is this anhydrous?
1 réponse-
This product is not considered anhydrous. The maximum allowable water content is 0.1% which is reported on the lot-specific Certificate of Analysis. Please see the link below to review the anhydrous DMSO products available:
https://www.sigmaaldrich.com/search/dmso-anhydrous?focus=products&page=1&perpage=30&sort=relevance&term=DMSO%20anhydrous&type=productUtile ?
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I ordered 10 ml glass vials, used 7 ml, Can I store it in centrifuge tubes? For how long can I use that?
1 réponse-
It may be best to store DMSO in amber glass vials for long term storage, as this compound is not compatible with many types of plastics. The compatibility of DMSO with various materials is listed below:
Compatible: LDPE, HDPE, polypropylene, PPCO(polypropylene copolymer), polymethylpentene, nylon, teflon FEP
Moderately compatible: polystyrene, ECTFE/ETFE
Incompatible: polysulfone, flexible and rigid PVC tubing, polycarbonateNote that DMSO is hygroscopic and light sensitive. The storage container selected must offer protection from light and moisture uptake. The product is assigned a firm expiration date. Please refer to the lot specific Certificate of Analysis to assume the material is within the expected shelf life.
Utile ?
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E' solo ad uso per ricerca o utilizzabile anche in diagnostica/human? Grazie
1 réponse-
This product is not suitable for diagnostic/human use.
Utile ?
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Is this product packaged in a bottle with a septa cap?
1 réponse-
No, this product is not packaged with a septa cap. It comes in a 125 ml amber glass bottle with a 28 mm solid white cap.
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Can I use it for PCR?
1 réponse-
While this product is tested specifically for use in cell culture, there is no reason to suspect that it would not perform well in PCR applications. The product is NOT tested for amplification, DNAse, or nuclease activity. Should this testing be important, see the link below to product options that include this testing:
D9170 DMSO PCR Reagent:
https://www.sigmaaldrich.com/product/sigma/d9170
D8418 DMSO Molecular Biology Tested
https://www.sigmaaldrich.com/US/en/product/sigma/d8418Utile ?
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what is the recommended re-test date & for how long can it be used beyond the expiry date mentioned in the COA?
1 réponse-
This product is assigned a firm expiration date of approximately 2 years. It is not assigned a retest date. The stability beyond the lot-specific expiration date is not determined and the quality of the product cannot be guaranteed. See the link below to review a sample Certificate of Analysis: https://www.sigmaaldrich.com/coa/SIGMA/D2650/RNBK5742.
Utile ?
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Where does DMSO come from?
1 réponse-
DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.
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