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Monoclonal Anti-Fibronectin antibody produced in mouse

clone IST-3, ascites fluid

Anti-MSF, Anti-FINC, Anti-GFND, Anti-LETS, Anti-SMDCF, Anti-FN, Anti-ED-B, Anti-GFND2, Anti-CIG, Anti-FNZ
Numéro MDL:

Niveau de qualité


Source biologique




Forme d'anticorps

ascites fluid

antibody product type

primary antibodies


IST-3, monoclonal

Poids mol.

antigen 240 kDa


15 mM sodium azide

species reactivity

bovine, human, rabbit, canine, goat, sheep, chicken


immunocytochemistry: suitable
immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:50 using cultured human fibroblasts
microarray: suitable
radioimmunoassay: suitable
western blot: suitable



N° d'accession UniProt

Expédié(e)(s) dans

dry ice

Temp. de stockage


Gene Information

human ... FN1(2335)

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Description générale

Fibronectin (FN) is an extracellular matrix protein composed of two nearly-identical disulfide-bound polypeptides with typical molecular weights of 220-280 kDa. Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma and antibodies to either form usually cross-react. Careful analysis of the FN molecule indicates that it contains several functionally and structurally distinct domains which may bind to cell surfaces and to a variety of molecules such as collagen, heparin, gelatin, fibrin and DNA. FN′s play an important role in diverse biological phenomena including cell adhesion, cell migration, hemostasis and thrombosis, wound healing and the ability to induce a more normal phenotype in transformed cells.
Monoclonal Anti-Human Fibronectin (mouse IgG1 iso-type) is derived from the IST-3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c immunized mice. Fibronectin (FN) is a multifunctional extracellular matrix protein comprising two nearly-identical disulfide-bound polypeptides. It is an adhesive glycoprotein. FN belongs to the ligand glycoprotein family and is is expressed in various cell types. Human fibronectin consists of five distinct domains that are referred to as heparin (Hep)-1/ fibrin (Fib)-1, gelatin (Gel), cell surface (Cell), Hep-2 and Fib-2, based on their affinity for Hep, Gel, Cell or Fib. The FN gene is mapped to human chromosome location 2q35.


Monoclonal Anti-Human Fibronectin, clone IST-3, recognizes an epitope located within the fourth type-three repeat of human plasma fibronectin.


human plasma fibronectin


Mouse monoclonal clone IST-3 anti-Human Fibronectin antibody has been used in:
  • enzyme linked immuno sorbent assay (ELISA)
  • western blotting
  • dot blot
  • radio immunoassay (RIA)
  • immunocytochemistry
  • immunohistochemistry
  • immunoprecipitation
  • immunofluorescence

Actions biochimiques/physiologiques

Fibronectin (FN) binds to cell surfaces and a variety of molecules, such as collagen, heparin, gelatin, fibrin, and DNA. It plays an important role in diverse biological phenomena including cell adhesion, cell migration, hemostasis, and thrombosis. FN is also involved in wound healing and stimulates a normal phenotype in transformed cells. Upregulation of FN leads to tumors, such as nasopharyngeal carcinoma (NPC), ovarian cancer, and head and neck squamous cell carcinoma.

Forme physique

The product is provided as ascites fluid containing 0.1% sodium azide as preservative.

Stockage et stabilité

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

Certificat d'analyse

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Certificat d'origine

Saisir un numéro de lot pour rechercher un certificat d'origine (COO).

Quotes and Ordering

Sahel N Abduljauwad et al.
Scientific reports, 9(1), 5935-5935 (2019-04-13)
Cancer metastasis results from the suppression of adhesion between cancer cells and the extracellular matrix, causing their migration from the primary tumor location and the subsequent formation of tumors in distant organs. This study demonstrates the potential use of nano-sized
Cellular distribution of invadopodia is regulated by nanometer scale surface
protein patterns
Ayaz GB, et al.
European Journal of Cell Biology, 673-684 (2017)
Sarah Calve et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 26(6), 2538-2545 (2012-03-15)
During forelimb regeneration in the newt Notophthalmus viridescens, the dynamic expression of a transitional matrix rich in hyaluronic acid, tenascin-C, and fibronectin controls muscle cell behavior in vivo and in vitro. However, the influence of extracellular matrix (ECM) remodeling on
Sebastian Müller et al.
Nature chemistry, 12(10), 929-938 (2020-08-05)
CD44 is a transmembrane glycoprotein linked to various biological processes reliant on epigenetic plasticity, which include development, inflammation, immune responses, wound healing and cancer progression. Although it is often referred to as a cell surface marker, the functional regulatory roles
Guillaume de Bonnecaze et al.
The Laryngoscope, 126(8), E278-E285 (2016-04-15)
Adipose derived stromal cells (ASCs) are abundant and easy to prepare. Such cells may be useful for treating severe vocal disturbance caused by acute vocal fold scars. Prospective animal experiments with controls. Twenty New-Zealand white rabbits were used in the

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