Merck
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F1804

Sigma-Aldrich

Monoclonal ANTI-FLAG® M2 antibody produced in mouse

1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

Synonym(s):
Anti-ddddk, Anti-dykddddk, flag antibody
NACRES:
NA.32

Niveau de qualité

200

Source biologique

mouse

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

antibody product type

primary antibodies

Clone

M2, monoclonal

Forme

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

Purifié(e)(s) par

affinity chromatography (affinity tag purification)

species reactivity

all

Concentration

1 mg/mL

Isotype

IgG1

Séquence immunogène

DYKDDDDK

Expédié(e)(s) dans

wet ice

Temp. de stockage

−20°C

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Description générale

The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.

Method of purification - Affinity tag purification

Spécificité

Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

Immunogène

FLAG; peptide sequence DYKDDDDK

Application

For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) , immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.

Western Blotting and EIA

Browse additional application references in our FLAG® Literature portal.

Forme physique

Contains 50% glycerol in phosphate buffered saline.

Notes préparatoires

Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk

Informations légales

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Code de la classe de stockage

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Articles

Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

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