Merck
All Photos(6)

F7378

Sigma-Aldrich

Fluorescein diacetate

used as cell viability stain

Synonym(s):
3,6-Diacetoxyfluoran, Di-O-acetylfluorescein
Empirical Formula (Hill Notation):
C24H16O7
CAS Number:
Molecular Weight:
416.38
Beilstein:
62575
Numéro EC:
Numéro MDL:
ID de substance PubChem:
NACRES:
NA.47

Niveau de qualité

Forme

powder

Pf

200-203 °C (lit.)

Solubilité

acetone: 25 mg/mL

ε (extinction coefficient)

≥86000 at 488-494 nm in 0.1 M NaOH

application(s)

diagnostic assay manufacturing
hematology
histology

Temp. de stockage

room temp

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccccc45)c1

InChI

1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3

InChI key

CHADEQDQBURGHL-UHFFFAOYSA-N

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Description générale

Fluorescein diacetate (FDA) is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA.

Application

Fluorescein diacetate has been used for the differentiation of live cells from dead cells.

Conditionnement

5, 10, 25, 100 g in glass bottle

Substrats

Lipase substrate

Code de la classe de stockage

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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