G1279

G 418 disulfate salt

suitable for plant cell culture, BioReagent

• Synonym(s): Antibiotic G418
• Empirical Formula (Hill Notation): C₂₀H₄₀N₄O₁₀ · 2H₂SO₄
• CAS Number: 108321-42-2
• Molecular Weight: 692.71
• Numéro MDL: MFCD00058314
• ID de substance PubChem: 24895058
• NACRES: NA.72

Properties

• Gamme de produits: BioReagent
• Niveau de qualité: 200
• Forme: powder
• Puissance: ≥720 μg per mg (Dried basis)
• technique(s): cell culture | plant: suitable
• Couleur: white to off-white
• Solubilité: 100 mg/mL (H₂O clear)
• Spectre d'activité de l'antibiotique: Gram-negative bacteria
• application(s): agriculture
• Mode d’action: protein synthesis | interferes
• Temp. de stockage: 2-8°C
• SMILES string: OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O
• InChI: 1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1
• InChI key: UHEPSJJJMTWUCP-NKCAIAFTSA-N

Description

Description générale

Chemical structure: aminoglycoside

Conditionnement

250 mg in glass bottle

1, 5 g in glass bottle

Application

G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.

Attention

The product is stable for three years when stored at 2-8°C.

Notes préparatoires

G418 powder is soluble in water at 100 mg/mL.

Safety Information

• Pictograms: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H317 - H334
• Conseils de prudence: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Classification des risques: Resp. Sens. 1 - Skin Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• WGK: WGK 3
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)