Merck
All Photos(4)

G1279

Sigma-Aldrich

G 418 disulfate salt

suitable for plant cell culture, BioReagent

Synonym(s):
Antibiotic G418
Empirical Formula (Hill Notation):
C20H40N4O10 · 2H2SO4
CAS Number:
Molecular Weight:
692.71
Numéro MDL:
ID de substance PubChem:
NACRES:
NA.72

Gamme de produits

BioReagent

Niveau de qualité

200

Forme

powder

Puissance

≥720 μg per mg (Dried basis)

technique(s)

cell culture | plant: suitable

Couleur

white to off-white

Solubilité

100 mg/mL (H2O clear)

Spectre d'activité de l'antibiotique

Gram-negative bacteria

application(s)

agriculture

Mode d’action

protein synthesis | interferes

Temp. de stockage

2-8°C

SMILES string

OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O

InChI

1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1

InChI key

UHEPSJJJMTWUCP-NKCAIAFTSA-N

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Description générale

Chemical structure: aminoglycoside

Conditionnement

250 mg in glass bottle
1, 5 g in glass bottle

Application

G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.

Attention

The product is stable for three years when stored at 2-8°C.

Notes préparatoires

G418 powder is soluble in water at 100 mg/mL.

Pictograms

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

11 - Combustible Solids

WGK

WGK 3

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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