Merck
All Photos(4)

K1377

Sigma-Aldrich

Kanamycin sulfate from Streptomyces kanamyceticus

powder, BioReagent, suitable for cell culture, suitable for plant cell culture

Synonym(s):
Kanamycin A, Kanamycin sulfate salt
Empirical Formula (Hill Notation):
C18H36N4O11 · H2O4S
CAS Number:
Molecular Weight:
582.58
Numéro EC:
Numéro MDL:
ID de substance PubChem:
NACRES:
NA.76

Source biologique

Streptomyces kanamyceticus

Niveau de qualité

200

Gamme de produits

BioReagent

Forme

powder

Puissance

≥750 μg per mg (dry basis)

technique(s)

cell culture | mammalian: suitable
cell culture | plant: suitable

Impuretés

≤5% kanamycin B

Couleur

white to off-white

Solubilité

H2O: 10-50 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 5 days.)

Spectre d'activité de l'antibiotique

Gram-negative bacteria
Gram-positive bacteria
mycobacteria
mycoplasma

application(s)

agriculture

Mode d’action

protein synthesis | interferes

SMILES string

OS(O)(=O)=O.NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C18H36N4O11.H2O4S/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17;1-5(2,3)4/h4-18,23-29H,1-3,19-22H2;(H2,1,2,3,4)/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-;/m1./s1

InChI key

OOYGSFOGFJDDHP-KMCOLRRFSA-N

Looking for similar products? Visit Product Comparison Guide

Description générale

Chemical structure: aminoglycoside
Kanamycin sulfate is a broad spectrum aminoglycoside-antibiotic derived from Streptomyces kanamyceticus. It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100μg/mL.

Application

Kanamycin sulfate from Streptomyces kanamyceticus has been used in tissue culture media.

Conditionnement

1, 5, 25 g in poly bottle

Actions biochimiques/physiologiques

Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding.

Mode of Resistance:Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.

Antimicrobial spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.

Attention

Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.

Notes préparatoires

Kanamycin sulfate is soluble in water at 50 mg/mL, yielding a clear solution. It is practically insoluble in alcohol, acetone, chloroform, ether and ethyl acetate. A 1% solution in water has a pH of 6.5 to 8.5. Sterile solutions can be prepared by a sterile filtration, through a .2μm filter.

Pictograms

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Conseils de prudence

Classification des risques

Repr. 1B

Code de la classe de stockage

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

More Documents

SDS

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service