L3022

LB Broth (Lennox)

Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

• Synonym(s): Lennox broth, Luria Bertani Broth
• NACRES: NA.85

Properties

• Niveau de qualité: 200
• Qualité: for molecular biology
• Stérilité: non-sterile
• Forme: powder
• Composition: NaCl, 5 g/L ; Tryptone, 10 g/L ; Yeast Extract, 5 g/L
• pH: 6.8-7.2(2% solution)
• application(s): food and beverages
• Temp. de stockage: room temp
• Adéquation: nonselective for Escherichia coli; nonselective for coliforms

Description

Description générale

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin^®.

Luria Broth is used for maintenance and propagation of Escherichia coli. The E. coli grow faster in Luria Broth because the tryptone and yeast supply essential growth factors that the E. coli would otherwise have to synthesize. Luria Broth also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.
It was used for resuspension of T. pyriformis cell fractions in a study of Trimastix fractionation of cellular extracts.

Conditionnement

250 g in poly bottle

1 kg in poly bottle

Caractéristiques et avantages

Lennox LB powder provides:

• Convenient small package to eliminate weighing
• Easy scale-up using larger package sizes
• A budget-friendly alternative to liquid
• Standard formulation

Notes préparatoires

1. Suspend 20 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121 °C.
To prepare Lennox L Broth: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1 M magnesium sulfate after autoclaving.

Informations légales

Zeocin is a registered trademark of Cayla Sarl

Safety Information

• Code de la classe de stockage: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Point d'éclair C: Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)