Merck
All Photos(2)

L3287

Sigma-Aldrich

Escort IV Transfection Reagent

Lipid reagent for transient and stable transfection of mammalian and insect cells.

Synonym(s):
Gene delivery
NACRES:
NA.25

Qualité

for molecular biology

Niveau de qualité

100

Forme

liquid (aqueous solution)

Utilisation

 mL sufficient for 160-500 transfections

Concentration

1 mg/mL

technique(s)

transfection: suitable

Temp. de stockage

2-8°C

Related Categories

Description générale

Escort IV is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into a wide variety of eukaryotic cell types.

Application

Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 4-16 μL Escort IV and 2 μg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. For a list of cells that have been successfully transfected using Escort IV, see the Transfection Reagent Selection Guide.

Caractéristiques et avantages

  • Suitable for stable and transient transfection
  • Optimized for a wide variety of cell lines
  • Low toxicity
  • Compatible with both serum and serum-free transfection protocols
  • Ideal for Sf9, Sf21 and S2 insect cells

Composants

Escort IV formulation:
1 mg/mL total lipid in water

Note the identity of the lipids used in Escort IV is confidential.

Attention

Do not freeze.

Principe

A stable complex is formed when Escort IV is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.

Informations légales

Escort is a trademark of Sigma-Aldrich Co. LLC

Code de la classe de stockage

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service