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M7279

Sigma-Aldrich

N,N′-Methylenebisacrylamide

powder, for molecular biology, suitable for electrophoresis, ≥99.5%

Synonyme(s):
BIS
Formule linéaire:
(CH2=CHCONH)2CH2
Numéro CAS:
Poids moléculaire:
154.17
Beilstein:
1706297
Numéro EC:
Numéro MDL:
ID de substance PubChem:
NACRES:
NB.22

Niveau de qualité

200

Qualité

for molecular biology

Essai/Dosage

≥99.5%

Forme

powder

technique(s)

electrophoresis: suitable

Impuretés

≤0.05% Free carboxylic acid (T)
≤50 mg/kg Total sulfur (as SO4 ) (ICP)

Conductivité

≤10 μmho (2% solution)

Traces d'anions

chloride (Cl-): ≤50 mg/kg

Traces de cations

Ca: ≤10 ppm
Cd: ≤5 ppm
Co: ≤5 ppm
Cr: ≤5 ppm
Cu: ≤5 ppm
Fe: ≤5 ppm
K: ≤50 ppm
Mg: ≤5 ppm
Mn: ≤5 ppm
Na: ≤50 ppm
Ni: ≤5 ppm
Pb: ≤5 ppm
Zn: ≤5 ppm

Absorption

≤0.2 at 290 at 1%

Activité étrangère

DNase and RNase, none detected

SMILES string

C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)

InChI key

ZIUHHBKFKCYYJD-UHFFFAOYSA-N

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Description générale

N,N′-Methylenebisacrylamide is commonly called as bis. It is used as a cross-linking agent in the preparation of polyacrylamide gels consisting of chains of acrylamide monomers. The concentration of N,N′-methylenebisacrylamide and acrylamide determines the pore size of the gel. The higher concentration of bis forms rigid gels with smaller pores due to more cross-linking leading to polymerization and vice versa.

Application

N,N′-Methylenebisacrylamide has been used:
  • as a crosslinker in the monomer solution to prepare N,N-dimethyl acrylamide (DMAAm)-based cryogel and acrylamide (AAm)-based cryogel matrix
  • as a crosslinker to prepare tough hydrogel network
  • in the preparation of acrylamide-bisacrylamide stock solution for the preparation of polyacrylamide gel

To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis.

Conditionnement

25, 100, 250 g in poly bottle

Pictograms

Skull and crossbonesHealth hazard

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Acute Tox. 3 Oral - Muta. 1B

Code de la classe de stockage

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificat d'analyse

Certificat d'origine

Depletion of hemoglobin and carbonic anhydrase from erythrocyte cytosolic samples by preparative clear native electrophoresis
Damici GM, et al.
Nature Protocols, 7(1), 36-36 (2012)
Fundamentals and Techniques of Biophysics and Molecular Biology (2018)
Ultrastretchable Conductor Fabricated on Skin-Like Hydrogel-Elastomer Hybrid Substrates for Skin Electronics
Kim SH, et al.
Advanced Materials, 30(26), 1800109-1800109 (2018)
Ashok Kumar et al.
Nature protocols, 5(11), 1737-1747 (2010-10-30)
In cell affinity chromatography, type-specific cell separation is based on the interaction between cell-surface receptors and an immobilized ligand on a stationary matrix. This protocol describes the preparation of monolithic polyacrylamide and polydimethylacrylamide cryogel affinity matrices that can be used
Ghazaleh Haghiashtiani et al.
Science advances, 6(35), eabb4641-eabb4641 (2020-09-15)
Minimally invasive surgeries have numerous advantages, yet complications may arise from limited knowledge about the anatomical site targeted for the delivery of therapy. Transcatheter aortic valve replacement (TAVR) is a minimally invasive procedure for treating aortic stenosis. Here, we demonstrate

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