Neurofilament 200, also known as neurofilament heavy polypeptide (NEFH), is encoded by the gene mapped to human chromosome 22q12.2. The encoded protein has a molecular mass of 200kDa and belongs to the group of type IV intermediate filaments which are key constituents of the neuronal cytoskeleton. NEFH contains C-terminal motifs involved in the formation of sidearm projections which cross-link the neurofilaments.
Shows a wide species cross-reactivity. Reacts with neurofilaments in the central and peripheral nervous system and localizes the neurofilament polypeptide.
Neurofilament 200 from bovine spinal cord.
Anti-neurofilament 200 antibody produced in rabbit has been used for immunolabeling.
Anti-neurofilament 200 antibody produced in rabbit has been used:
- for detection of neurofilaments using immunohistological staining
- in human astrocytoma cell line
- in post-mortem brain sections
- in human cremaster muscles
- for detection of neurofilaments by immunofluorescence staining in lumbar spinal cord sections of mice
Neurofilament 200 plays a vital role in cytoarchitecture organization. Downregulated expression of the gene has been observed in schizophrenia patients. Loss of gene expression is associated with the development of prostate carcinoma. Mutation in the gene leads to sporadic amyotrophic lateral sclerosis (ALS).
Neurofilament 200 assembly during development is critical for normal functioning of neuronal cells. Mutations in gene encoding neurofilaments is associated with neurological disorder called the Charcot–Marie–Tooth.
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Stockage et stabilité
Store at -20°C. For continuous use, the product may be stored at 2-8°C for up to one month. For extended storage, solution may be frozen in working aliquots at –20°C. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
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