N4645

4-Nitrophenyl phosphate disodium salt hexahydrate

powder, BioReagent, suitable for cell culture, ≥97%

• Synonym(s): p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate
• Linear Formula: O₂NC₆H₄OP(O)(ONa)₂ · 6H₂O
• CAS Number: 333338-18-4
• Molecular Weight: 371.14
• Beilstein: 5324661
• Numéro EC: 224-246-5
• Numéro MDL: MFCD00066288
• ID de substance PubChem: 24897708
• NACRES: NA.75

Properties

• Source biologique: synthetic (organic)
• Niveau de qualité: 200
• Gamme de produits: BioReagent
• Essai/Dosage: ≥97%
• Forme: powder
• technique(s): cell culture | mammalian: suitable
• Solubilité: H₂O: 100 mg/mL
• Temp. de stockage: −20°C
• SMILES string: O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
• InChI: 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
• InChI key: KAKKHKRHCKCAGH-UHFFFAOYSA-L

Description

Description générale

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Application

4-Nitrophenyl phosphate disodium salt hexahydrate has been used:

• as a component in diethanolamine buffer to detect alkaline phosphatase-conjugated antibodies in enzyme linked immunosorbent assay (ELISA)[1]
• to treat adherent cells in adhesion assay[2]
• as a phosphatase substrate in in vitro phosphatase assay[3] in substrate buffer for ELISA[4]

Conditionnement

1, 5 g in poly bottle

Actions biochimiques/physiologiques

p-Nitrophenyl phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in enzyme linked immunosorbent assay (ELISA) procedures. It is the substrate of choice in alkaline phosphatase systems due to its high sensitivity. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. This product is tested for use in cell culture applications. 4-nitrophenyl phosphate (pNPP), a small-molecule substrate can be used to study on alkaline phosphatases, acid phosphatases etc.[5]

Safety Information

• Code de la classe de stockage: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Point d'éclair C: Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)