N6386

Neomycin trisulfate salt hydrate

powder, BioReagent, suitable for cell culture

• Synonym(s): Antibiotic 10676, Neomycin B
• Empirical Formula (Hill Notation): C₂₃H₄₆N₆O₁₃ · 3H₂SO₄ · xH₂O
• CAS Number: 1405-10-3
• Molecular Weight: 908.88 (anhydrous basis)
• Numéro EC: 215-773-1
• ID de substance PubChem: 24897673
• NACRES: NA.76

Properties

• Source biologique: Streptomyces sp.
• Niveau de qualité: 200
• Gamme de produits: BioReagent
• Forme: powder
• Puissance: ≥600 μg neomycin per mg (Dried basis)
• Conditionnement: pkg of 100 g; pkg of 25 g; pkg of 5 g
• technique(s): cell culture | mammalian: suitable
• Couleur: white to yellow
• Solubilité: H₂O: 50 mg/mL (As a stock solution. Stock solutions should be filter sterilized and stored at 2-8°C. Stable at 37°C for 5 days.)
• Spectre d'activité de l'antibiotique: Gram-negative bacteria; Gram-positive bacteria
• Mode d’action: protein synthesis | interferes
• Temp. de stockage: 2-8°C
• SMILES string: O.OS(O)(=O)=O.OS(O)(=O)=O.OS(O)(=O)=O.NC[C@@H]1O[C@H](O[C@H]2[C@@H](O)[C@@H](O[C@@H]2CO)O[C@@H]3[C@@H](O)[C@H](N)C[C@H](N)[C@H]3O[C@H]4O[C@H](CN)[C@@H](O)[C@H](O)[C@H]4N)C(N)[C@@H](O)[C@@H]1O
• InChI: 1S/C23H46N6O13.3H2O4S.H2O/c24-2-7-13(32)15(34)10(28)21(37-7)40-18-6(27)1-5(26)12(31)20(18)42-23-17(36)19(9(4-30)39-23)41-22-11(29)16(35)14(33)8(3-25)38-22;3*1-5(2,3)4;/h5-23,30-36H,1-4,24-29H2;3*(H2,1,2,3,4);1H2/t5-,6+,7-,8+,9-,10-,11?,12+,13-,14-,15-,16-,17-,18-,19-,20-,21-,22-,23+;;;;/m1..../s1
• InChI key: WHAGUNPVKDUVFV-QGTTWHFQSA-N

Description

Description générale

Chemical structure: aminoglycoside

Application

Neomycin trisulfate salt hydrate has been used:

• to determine Netrin1- encoding gene (Ntn1) protective effects against aminoglycoside antibiotics in cochlear explants
• for Escherichia coli antibiotic susceptibility test
• in bone marrow transplantation of mice

Conditionnement

1, 5, 10, 25, 100 g in glass bottle

Actions biochimiques/physiologiques

Neomycin Trisulfateis an aminoglycoside antibiotic, which is produced by Streptomyces containing a minimum of 85% neomycin B.It is used to study the cytotoxic side effects of antibiotics, platelet-derived growth factor responses in certain fibroblasts and extraction of nuclear phosphatidylinositol 4,5-bisphosphate-interacting proteins. Neomycin trisulfate functions as a selection agent for prokaryotic cells transformed using the neo selectable marker gene. This product is recommended for use in cell culture applications at 5 mL/L.

Mode of action: This product acts by binding to the 30S and 50S subunits, causing miscoding and inhibiting initiation and elongation during protein synthesis. Neomycin also blocks voltage-sensitive Ca²⁺ channels without affecting the Na⁺/Ca²⁺ antiporter in neurons.

Antimicrobial spectrum: Neomycin acts against both gram-positive and gram-negative bacteria.

Attention

Stock solutions should be filter sterilized and stored at 2-8°C, and are stable at 37°C for 5 days. A 1 mg/mL neomycin solution in .1 M phosphate buffer, pH 8.0 should be used within 14 days when stored at 0-5°C. Solutions should be protected from light or moisture.

Notes préparatoires

Neomycin sulfate is soluble in H₂O at 50 mg/mL, yielding a clear solution.

Autres remarques

Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place.

Safety Information

• Pictograms: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H317 - H334
• Conseils de prudence: P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312
• Classification des risques: Resp. Sens. 1 - Skin Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Point d'éclair C: Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Faceshields, Gloves