N7653

p-Nitrophenyl Phosphate Liquid Substrate System

liquid

• Synonym(s): 4-Nitrophenyl phosphate disodium salt solution, pNPP
• Linear Formula: O₂NC₆H₄OP(O)(ONa)₂
• CAS Number: 4264-83-9
• Molecular Weight: 263.05
• Numéro MDL: MFCD00007319
• ID de substance PubChem: 24897835
• NACRES: NA.83

Properties

• Forme: liquid
• Niveau de qualité: 200
• Expédié(e)(s) dans: wet ice
• Temp. de stockage: −20°C
• SMILES string: [Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
• InChI: 1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2
• InChI key: VIYFPAMJCJLZKD-UHFFFAOYSA-L

Description

Description générale

The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.[1]

Application

p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase[2] in alkaline phosphatase assay.[3]

Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Conditionnement

100 mL in glass bottle

Actions biochimiques/physiologiques

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol.[4] It is a common chromogenic substrate for alkaline phosphatase.[5]

Attention

Stable at least one year when stored below 0 °C.

Forme physique

Ready-to-use.

Safety Information

• Pictograms: GHS05,GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H290 - H315 - H318 - H361fd - H373
• Conseils de prudence: P202 - P234 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Classification des risques: Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral
• Code de la classe de stockage: 8B - Non-combustible, corrosive hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Point d'éclair C: Not applicable