Merck
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P7998

Sigma-Aldrich

Alkaline Phosphatase Yellow (pNPP) Liquid Substrate System for ELISA

ready to use solution

Synonym(s):
Alkaline phosphatase substrate solution (pNPP)
eCl@ss:
42010105
NACRES:
NA.83

Niveau de qualité

200

Forme

liquid

Temp. de stockage

2-8°C

Description générale

The product is ready to use buffered alkaline phosphatase substrate consist of p-nitrophenylphosphate (pNPP). This is the advanced substrate system contains insoluble stabilizer that enhances the stability and reactivity as well as reduces the base-line absorption. It produces a soluble end product, hence not recommended for blotting or histochemistry applications.

Application

Develops a soluble yellow reaction product that may be read at 405 nm. For endpoint assays, the reaction may be stopped with 3 N NaOH. Recommended for ELISA (microwell) procedures; not recommended for membrane applications.

Attention

 Stable at least one year at 2-8 °C.

Forme physique

Ready to use

Notes préparatoires

Contains p-nitrophenyl phosphate, a substrate for alkaline phosphatase.

Pictograms

CorrosionHealth hazard

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Eye Dam. 1 - Skin Irrit. 2 - STOT RE 2

Code de la classe de stockage

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

Certificate of Analysis

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Certificate of Origin

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