Merck
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P9187

Sigma-Aldrich

SIGMAFAST OPD

tablet

Synonym(s):
OPD Peroxidase Substrate
NACRES:
NA.83

Forme

tablet

Niveau de qualité

200

Solubilité

water: 0.5 tablet/10 mL, clear, colorless

Temp. de stockage

2-8°C

Application

SIGMAFAST OPD (o-Phenylenediamine dihydrochloride) tablets have been developed for use as a soluble substrate for the detection of peroxidase activity in enzyme immunoassays (EIA). OPD is the EIA substrate of choice as it exhibits high sensitivity. EIA applications utilizing OPD may be read in timed assays or stopped with dilute acid solutions for delayed readings.
SIGMAFAST OPD has been used in saliva- virus-like particles (VLP) binding assay. It has also been used to develop enzyme linked immunosorbent assay (ELISA) plates for the detection of rituximab in rat serum samples.

Caractéristiques et avantages

SIGMAFAST OPD Tablets require no additional buffers or steps to prepare an active substrate solution.

Attention

This substrate is hygroscopic and light sensitive

Reconstitution

Each tablet set dissolved in 20 mL deionized water yields a ready-to-use buffered solution containing OPD and urea hydrogen peroxide.
One OPD tablet and one urea hydrogen peroxide/buffer tablet, dissolved in 20 ml of water, provides 20 ml of ready-to-use substrate. When the tablet is dissolved in this manner, the final concentrations will be 0.4 mg/ml OPD, 0.4 mg/ml urea hydrogen peroxide, and 0.05 M phosphate-citrate, pH 5.0. Tablets are individually packaged in foil packets.

Informations légales

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Skin Sens. 1 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

WGK

WGK 1

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

SDS

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