Endotoxin-free products are all typically filtered with a 0.1 micron pore size filter. Sterile filtered products typically have a 0.2 or 0.22 pore size filter.
R8758
Milieu RPMI-1640
With ʟ-glutamine, sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
RPMI-1640 Medium
Synonyme(s) :
Roswell Park Memorial Institute 1640 medium
Se connecter pour consulter les tarifs organisationnels et contractuels.
Sélectionner une taille de conditionnement
Changer de vue
| Conditionnement | Référence | Disponibilité | Prix |
|---|
A propos de cet article
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderNom du produit
Milieu RPMI-1640, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: yes
phenol red: yes
sodium pyruvate: no
HEPES: no
L-glutamine: yes
shipped in
ambient
storage temp.
2-8°C
General description
Le milieu RPMI 1640 a été mis au point par Moore et ses collaborateurs, au Roswell Park Memorial Institute (RPMI), en 1966. Version modifiée du milieu 5A de McCoy, il a été formulé pour permettre la culture de cellules lymphoblastoïdes en suspension, mais on sait depuis qu'il convient aussi à une grande diversité de cellules adhérentes. Initialement conçu pour être utilisé avec un supplément à base de sérum, le milieu RPMI 1640 s'est révélé adapté à la culture sans sérum de différentes lignées cellulaires. Il est aussi couramment employé dans les protocoles de fusion ainsi que pour la croissance de cellules hybrides. Ce milieu convient à la culture de leucocytes normaux et néoplasiques humains.
Application
1 of 1
Cet article | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form liquid | form liquid | form liquid | form liquid |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| components NaHCO3: yes | components sodium pyruvate: no | components sodium pyruvate: no | components phenol red: no |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
Still not finding the right product?
Explore all of our products under Milieu RPMI-1640
Classe de stockage
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Protocoles
Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.
Preparation for biodegradable nanoparticles and their use in transfection protocols .
Step-by-step protocol for ADCC assays using cryopreserved NK effector cells including tips and tricks, assay development, and data analysis methods.
Contenu apparenté
Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.
Learn how to use our cell culture tested, ready-to-use MilliShot™ single dose antibiotic solutions, conveniently packaged in one-time use vials.
Anders Abildgaard et al.
Psychoneuroendocrinology, 79, 40-48 (2017-03-05)
The gut microbiota has recently emerged as an important regulator of brain physiology and behaviour in animals, and ingestion of certain bacteria (probiotics) therefore appear to be a potential treatment for major depressive disorder (MDD). However, some conceptual and mechanistical
Katarzyna A Podyma-Inoue et al.
Cancer genomics & proteomics, 13(6), 443-452 (2016-11-04)
Heparan sulfate proteoglycans (HSPGs)-dependent endocytic events have been involved in glioma progression. Thus, comprehensive understanding of the intracellular trafficking complexes formed in presence of HSPGs would be important for development of glioma treatments. Subcellular fractionation was used to separate vesicles
E L Davies et al.
Clinical and experimental immunology, 145(1), 183-189 (2006-06-24)
An increasing number of cell types, including peripheral blood mononuclear cells (PBMCs), have been demonstrated to release heat shock proteins (Hsps). In this paper we investigate further the hypothesis that Hsps are danger signals. PBMCs and Jurkat cells released Hsp70
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| R8758-1L | 04061838259981 |
| R8758-500ML | 04061838259998 |
| R8758-100ML | 04061838259974 |
| R8758-2L | 04065266055030 |
| R8758-6X500ML | 04061838260000 |
| R8758-24X500ML | 04061836823863 |
| R8758-6X1L | 04061836823870 |
-
When the medium waas sterile-filtered, which pore size was used?
1 réponse-
Utile ?
-
-
Good Morning, does RPMI medium R8758 which contains sodium bicarbonate, affect cell suspension culture of megakaryoblastic cell line DAMI? I mean, sodium bicarbonate can significantly modify cell behavior or his effects can be tolerated?
1 réponse-
This product has not been validated for use with the megakaryoblastic cell line DAMI. However, literature indicates compatibility with this cell line. The following citation may be of interest: PLoS One. 2020 Apr 7;15(4):e0230507.
Utile ?
-
-
I bought this product and realized that it does not contain Hepes. Can I add hepes?
1 réponse-
This product utilizes a bicarbonate buffering system. However, there are no anticipated problems with the addition of HEPES. We do offer RPMI formulations containing both sodium bicarbonate and HEPES, such as Products R5886 & R7638.
Please see the RPMI formulation tables at the link below: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/203/777/r8758for.pdf
Utile ?
-
-
Considering that I have to use an RPMI medium containing 4mM of l-Glutamine and 10mM of non-essential amino acids, can I use the product R8748 without adding anything?
1 réponse-
The information you are requesting can be supplied by our Technical Service team who can assist you further. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, and click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.
Utile ?
-
-
buenos dias, llevo años usando vuestro producto RPMI 1640, y me gustaría saber la composición del medio para ver si hay algun metal/producto que no puede utilizar con un suplemento que quiero introducir. Muchas gracias
1 réponse-
Please see the link below to review the RPMI formulation tables. This product is listed in the second column of page 2:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/203/777/r8758for.pdfUtile ?
-
-
Can Product R8758, RPMI-1640 Medium, be frozen?
1 réponse-
We do not recommend freezing cell culture media. A precipitate may form upon thawing. Also, this can cause a loss of amino acids, which may yield low cell growth.
Utile ?
-
-
Does Product R8758, RPMI-1640 Medium, contain glucose?
1 réponse-
RPMI contains glucose at 2 g/L. The complete formulation of RPMI and other classical media formulations can be found on the Cell Culture Web Page.
Utile ?
-
-
What is the Department of Transportation shipping information for this product?
1 réponse-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Utile ?
-
-
Can I add the glutamine dipeptide to Product R8758, RPMI-1640 Medium?
1 réponse-
This formulation of RPMI already contains L-glutamine at 0.3 g/L. It does not require further supplementation with L-glutamine or the L-glutamine dipeptide (Product No. G8541).
Utile ?
-
-
Does Product R8758, RPMI-1640 Medium, contain phenol red?
1 réponse-
This media contains phenol red. The formulation of RPMI and other classical media can be found on the Cell Culture Web Page.
Utile ?
-