SHC014V

Sigma-Aldrich

MISSION® pLKO.1-puro-UbC-TurboGFP Positive Control Transduction Particles

Contains a gene encoding TurboGFP, under the control of the UbC promoter

Synonyme(s):
MISSION TurboGFP Control Transduction Particles
NACRES:
NA.51

Niveau de qualité

200

Gamme de produits

MISSION®

Concentration

≥1x106 VP/ml (via p24 assay)

Application(s)

capture ELISA: 106 TU/mL using p24

Expédié(e)(s) dans

dry ice

Temp. de stockage

−70°C

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Description générale

Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit
sigma.com/shrna.

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.
When conducting experiments using MISSION shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. Silencing of the CMV promoter can be a problem in some cell types. For these cells, the ubiquitin promoter (UbC) can be a viable alternative. The MISSION UbC-TurboGFP Control Transduction Particles are a positive control to monitor transduction efficiency for applications in which an alternate promoter is necessary. The MISSION UbC-TurboGFP Control Transduction Particles are produced from the sequence-verified 9092 base pair lentivirus plasmid, pLKO.1-puro-UbC-TurboGFP (SHC014).

This construct aids in interpretation of experimental design and results by providing a green fluorescent protein marker for monitoring success in transduction of your cells of interest.

Informations légales

MISSION is a registered trademark of Sigma-Aldrich Co. LLC
TurboGFP is a trademark of Evrogen Co.

RID ADR

UN 3245 9

WGK Allemagne

WGK 3

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

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