SRP3055

Sigma-Aldrich

Heregulin beta -1 human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonyme(s):
Neuregulin1, NRG1-b1
NACRES:
NA.32

Source biologique

human

Recombinant

expressed in E. coli

Essai/Dosage

≥98% (HPLC)
≥98% (SDS-PAGE)

Forme

lyophilized

Puissance

≤0.5 ng/mL

Poids mol.

7.5 kDa

Conditionnement

pkg of 50 μg

Application(s)

cell culture | mammalian: suitable

Impuretés

<0.1 EU/μg endotoxin, tested

Couleur

white to off-white

N° d'accession UniProt

Expédié(e)(s) dans

wet ice

Temp. de stockage

−20°C

Gene Information

human ... NRG1(3084)

Catégories apparentées

Description générale

Neuregulin/Heregulin is a family of structurally related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). HRG1-β1 (heregulin-β1, also referred to as NRG1-β1) contains an Ig (immunoglobulin) domain and an EGF (epidermal growth factor)-like domain that is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. The gene HRG1-β1 is mapped to human chromosome 8p12. Recombinant human Heregulin-β1 is a 7.5 kDa polypeptide consisting of only the EGF domain of heregulin-β1 (65 amino acid residues).

Application

Heregulin-β1 human has been used in the culture medium for the maintenance of Schwann cells. It has been used to study P-Rex1 (PI(3,4,5)P3-dependent Rac exchanger 1)-driven fibroblast invasiveness.
Heregulin beta -1 human has been used to enhance proliferation of human dermal fibroblasts (hDFs).

Actions biochimiques/physiologiques

To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 (neuregulin1) gene. Proteolytic processing of the extracellular domain of the transmembrane NRG1 isoforms release soluble growth factors. HRG1-β1 (heregulin-β1, also referred to as NRG1-β1) binding to receptor tyrosine kinases erb3 and erb4 induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity, which leads to tyrosine phosphorylation. Although HRG1-β1 biological effects is still unclear, it has been found to promote motility and invasiveness of breast cancer cells which may also involve up-regulation of expression and function of the autocrine motility-promoting factor (AMF).

Séquence

SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKHLGIE FMEAE

Forme physique

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

RID ADR

NONH for all modes of transport

WGK Allemagne

WGK 3

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

Certificat d'analyse
Certificat d'origine
Enhancement of wound healing efficiency mediated by artificial dermis functionalized with EGF or NRG1
Yoon D, et al.
Biomedical Materials (Bristol, England), 13(4), 045007-045007 (2018)
Neuregulins: functions, forms, and signaling strategies.
Falls DL
Experimental Cell Research, 284, 14-30 (2003)
Control of peripheral nerve myelination by the beta-secretase BACE1.
Willem M
Science, 314, 664-666 (2006)
Neuregulins and cancer.
Montero JC
Clinical Cancer Research, 14, 3237-3241 (2008)
Heregulin-induced activation of ErbB3 by EGFR tyrosine kinase activity promotes tumor growth and metastasis in melanoma cells.
Ueno Y
International Journal of Cancer. Journal International Du Cancer, 123, 340-347 (2008)

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