SRP3179

Sigma-Aldrich

TSG human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

Synonyme(s):
Twisted Gastrulation Protein
NACRES:
NA.32

Source biologique

human

Recombinant

expressed in E. coli

Essai/Dosage

≥95% (HPLC)
≥95% (SDS-PAGE)

Forme

lyophilized

Puissance

≤2.0 μg/mL ED50

Poids mol.

22.2 kDa

Conditionnement

pkg of 50 μg

Application(s)

cell culture | mammalian: suitable

Impuretés

<0.1 EU/μg endotoxin, tested

Couleur

white

N° d'accession UniProt

Expédié(e)(s) dans

wet ice

Temp. de stockage

−20°C

Gene Information

human ... TWSG1(57045)

Description générale

Twisted gastrulation protein (TSG) is a secreted bone morphogenetic protein (BMP)-binding protein structurally related to the BMP antagonists chordin and noggin. The gene encoding TSG is localized on human chromosome 18p11.22. Recombinant human TSG is a 199 amino acid 22.2kDa protein containing the BMP/TGFb binding portion of the full length TSG protein.

Actions biochimiques/physiologiques

Twisted gastrulation protein (TSG) can inhibit bone morphogenetic protein (BMP) activity by binding directly to BMP proteins, and can act either as a BMP4 agonist or antagonist (depending on the specific biochemical environment) by binding to the BMP4/chordin complex.

Séquence

MCNKALCASD VSKCLIQELC QCRPGEGNCS CCKECMLCLG ALWDECCDCV GMCNPRNYSD TPPTSKSTVE ELHEPIPSLF RALTEGDTQL NWNIVSFPVA EELSHHENLV SFLETVNQPH HQNVSVPSNN VHAPYSSDKE HMCTVVYFDD CMSIHQCKIS CESMGASKYR WFHNACCECI GPECIDYGSK TVKCMNCMF

Forme physique

Lyophilized from 10 mM Sodium Acetate, pH 6.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

RID ADR

NONH for all modes of transport

WGK Allemagne

WGK 3

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

Certificat d'analyse
Certificat d'origine
Andrea Wills et al.
Developmental biology, 289(1), 166-178 (2005-12-03)
In the developing vertebrate embryo, proper dorsal-ventral patterning relies on BMP antagonists secreted by the organizer during gastrulation. The BMP antagonist chordin has a complex interaction with BMPs that is governed in part by its interaction with the secreted protein...
Minimal evidence for a direct involvement of twisted gastrulation homolog 1 (TWSG1) gene in human holoprosencephaly
Kauvar EF
Molecular Genetics and Metabolism (2011)
The evolutionarily conserved BMP-binding protein Twisted gastrulation promotes BMP signalling
Michael O
Nature (2000)

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