SRP3278

Sigma-Aldrich

ANGIOPOEITIN-2 human

recombinant, expressed in CHO cells, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonyme(s):
ANGPT2

Source biologique

human

Recombinant

expressed in CHO cells

Essai/Dosage

≥95% (HPLC)
≥95% (SDS-PAGE)

Forme

lyophilized

Poids mol.

60.0-70.0 kDa

Conditionnement

pkg of 20 μg

Impuretés

<0.1 EU/μg endotoxin, tested

Couleur

white to off-white

N° d'accession UniProt

Expédié(e)(s) dans

wet ice

Temp. de stockage

−20°C

Gene Information

human ... ANGPT2(1285)

Catégories apparentées

Description générale

The signaling interactions of ANG-1, ANG-2 and Tie2, along with less characterized ANG-3 and ANG-4, are required for embryonic and adult angiogenesis.  Physiologically, ANG-1 and ANG-2 are associated with sprouting, tube formation, and structural integrity of newly formed blood vessels.  Mature human ANG-2 is a secreted protein containing 480 amino acid residues.  ANG-2 is composed of an alpha helix rich “coiled coil” N-terminal domain and fibrinogen like C-terminal domain.

Actions biochimiques/physiologiques

ANG-2 binds to the endothelial cell specific receptor Tie2, but, in contrast to ANG-1 does not induce tyrosine phosphorylation.  Consequently, ANG-2 modulates ANG-1 activation of Tie2 and, depending on the physiological and biochemical environment, can act either as a n agonist or antagonist of Tie2 induced angiogenesis.  ANG-2 exists predominantly in the form of a disulfide-linked dimer. Recombinant human ANG-2 is a C-terminal histidine tagged glycoprotein which migrates with an apparent molecular mass of 60.0 – 70.0 kDa by SDS-PAGE under reducing conditions. Sequencing analysis shows an N-terminal sequence starting with residue 68 (D) of the ANG-2 precursor protein.

Forme physique

Lyophilized from 10 mM Sodium Phosphate, pH 8.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

RID ADR

NONH for all modes of transport

WGK Allemagne

3

Certificat d'analyse
Certificat d'origine
I Kim et al.
The Journal of biological chemistry, 275(24), 18550-18556 (2000-04-15)
Angiopoietin-2 (Ang2) is a naturally occurring antagonist of angiopoietin-1 (Ang1) that competes for binding to the Tie2 receptor and blocks Ang1-induced Tie2 autophosphorylation during vasculogenesis. Using the polymerase chain reaction, we isolated a cDNA encoding a novel shorter form of...

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