SRP5189

Sigma-Aldrich

HSP40, GST tagged human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonyme(s):
HSPF1, DNAJB1
Numéro CAS:
NACRES:
NA.32

Source biologique

human

Recombinant

expressed in E. coli

Essai/Dosage

≥70% (SDS-PAGE)

Forme

buffered aqueous glycerol solution

Poids mol.

~65 kDa

Application(s)

cell analysis: suitable

N° d'accession NCBI

Expédié(e)(s) dans

dry ice

Temp. de stockage

−70°C

Gene Information

human ... DNAJB1(3337)

Description générale

Heat shock protein 40 (HSP40) is synthesized in cells in response to heat shock and other metabolic stresses and provide a transient state of thermotolerance. HSP40 is localized faintly throughout the cell in non-heat-shocked cells and accumulates in the nuclei and nucleoli in heat-shocked cells. The intracellular localization of HSP40 is very similar to that of HSP70.The DNAJC8 (DnaJ heat shock protein family (Hsp40) member C8) gene is located on human chromosome 1p35.3. The protein is predominantly expressed in human lens epithelial (HLE) cells.

Actions biochimiques/physiologiques

Studies have indicated that DnaJ heat shock protein family (Hsp40) member C8 (DNAJC8) represses the aggregation of polyQ-containing protein in a model of Machado-Joseph disease, also referred to as spinocerebellar ataxia type 3 (SCA3). Hsp40 has its primary function to induce ATPase (adenosine triphosphatase) action of chaperones Hsp70s. Hsp40 protein is also associated with the translation and structural modification of proteins, mediating its folding and unfolding. It is also involved in protein translocation and degradation. Overexpression of HSP40 reduces aggregate formation and suppresses apoptosis in a neuronal cell model of spinal and bulbar muscular atrophy (inherited neurodegenerative diseases caused by polyglutamine expansion).

Forme physique

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Notes préparatoires

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

RID ADR

NONH for all modes of transport

WGK Allemagne

WGK 1

Certificat d'analyse
Certificat d'origine
The diversity of the DnaJ/Hsp40 family, the crucial partners for Hsp70 chaperones
Qiu XB, et al.
Cellular and molecular gastroenterology and hepatology, 63(22), 2560-2570 (2006)
FOXE3 contributes to Peters anomaly through transcriptional regulation of an autophagy-associated protein termed DNAJB1
Khan SY, et al.
Nature Communications, 7(4), 10953-10953 (2016)
Y Kobayashi et al.
The Journal of biological chemistry, 275(12), 8772-8778 (2000-03-18)
Spinal and bulbar muscular atrophy (SBMA) is one of a group of human inherited neurodegenerative diseases caused by polyglutamine expansion. We have previously demonstrated that the SBMA gene product, the androgen receptor protein, is toxic and aggregates when truncated. Heat...
Norio Iizuka et al.
International journal of cancer, 107(4), 666-672 (2003-10-02)
Although the physiological actions of many herbs are gradually being elucidated at the molecular level, it remains unclear how individual components of herbs contribute to their biological activities. In the present study, the antiproliferative activity of Coptidis rhizoma, a medicinal...
H Hattori et al.
Cell structure and function, 17(1), 77-86 (1992-02-01)
We earlier discovered a novel 40-kDa protein (hsp40) induced by heat shock and other stresses in mammalian and avian cells. In this report, we purified the hsp40 in HeLa cells, using modified two-dimensional gel electrophoresis, and determined the amino terminal...

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