Merck
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SRP5189

Sigma-Aldrich

HSP40, GST tagged human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):
DNAJB1, HSPF1
CAS Number:
NACRES:
NA.32

Source biologique

human

Recombinant

expressed in E. coli

Essai/Dosage

≥70% (SDS-PAGE)

Forme

buffered aqueous glycerol solution

Poids mol.

~65 kDa

N° d'accession NCBI

application(s)

cell analysis

Expédié(e)(s) dans

dry ice

Temp. de stockage

−70°C

Gene Information

human ... DNAJB1(3337)

Description générale

Heat shock protein 40 (HSP40) is synthesized in cells in response to heat shock and other metabolic stresses and provide a transient state of thermotolerance. HSP40 is localized faintly throughout the cell in non-heat-shocked cells and accumulates in the nuclei and nucleoli in heat-shocked cells. The intracellular localization of HSP40 is very similar to that of HSP70.The DNAJC8 (DnaJ heat shock protein family (Hsp40) member C8) gene is located on human chromosome 1p35.3.[1] The protein is predominantly expressed in human lens epithelial (HLE) cells.[2]

Actions biochimiques/physiologiques

Studies have indicated that DnaJ heat shock protein family (Hsp40) member C8 (DNAJC8) represses the aggregation of polyQ-containing protein in a model of Machado-Joseph disease, also referred to as spinocerebellar ataxia type 3 (SCA3).[1] Hsp40 has its primary function to induce ATPase (adenosine triphosphatase) action of chaperones Hsp70s. Hsp40 protein is also associated with the translation and structural modification of proteins, mediating its folding and unfolding. It is also involved in protein translocation and degradation.[3] Overexpression of HSP40 reduces aggregate formation and suppresses apoptosis in a neuronal cell model of spinal and bulbar muscular atrophy (inherited neurodegenerative diseases caused by polyglutamine expansion).

Forme physique

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Notes préparatoires

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Code de la classe de stockage

10 - Combustible liquids

WGK

WGK 1

Certificate of Analysis

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Certificate of Origin

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