SRP6135

Sigma-Aldrich

Ubiquitin-K27R human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

NACRES:
NA.32

Source biologique

human

Recombinant

expressed in E. coli

Essai/Dosage

≥95% (SDS-PAGE)

Forme

liquid

Poids mol.

8.592 kDa

Conditionnement

pkg of 500 μg

N° d'accession NCBI

Expédié(e)(s) dans

dry ice

Temp. de stockage

−70°C

Gene Information

human ... RPS27A(6233)

Description générale

Ubiquitin is a small polypeptide that can be conjugated via its C-terminus to amine groups of lysine residue on target proteins. This conjunction is referred to as monoubiquitylation. Additional ubiquitin moieties can be subsequently conjugated to this initial ubiquitin, utilizing any one of the seven lysine residues on the surface of ubiquitin. The formation of these ubiquitin chains is referred to as polyubiquitylation. This tag-free recombinant form of human ubiquitin is engineered to have a single substitution of arginine for lysine at position 27. Covalent attachment of ubiquitin to other proteins serves various functions, but its major role is to target cellular proteins for destruction. Cellular components that activate, transfer, remove, or simply recognize ubiquitin number in the hundreds, perhaps even in the thousands. In light of this complexity the ubiquitin pathway is ideal for a systems biology approach. Ubiquitin plays a very important role in regulated non-lysosomal ATP dependent protein degradation. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes.

Forme physique

4 mg/mL in 20 mM Tris, pH 7.5, 0.15 M NaCl and 10% glycerol.

Notes préparatoires

Centrifuge the vial prior to opening.

RID ADR

NONH for all modes of transport

WGK Allemagne

WGK 3

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

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