Merck
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T5168

Sigma-Aldrich

Monoclonal Anti-α-Tubulin antibody produced in mouse

enhanced validation

ascites fluid, clone B-5-1-2

Synonym(s):
Alpha Tubulin Antibody Sigma, Anti Tubulin Sigma, Tubulin Antibody Sigma, Tubulin Antibody Sigma - Monoclonal Anti-α-Tubulin antibody produced in mouse
Numéro MDL:
NACRES:
NA.41

Source biologique

mouse

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

ascites fluid

antibody product type

primary antibodies

Clone

B-5-1-2, monoclonal

Poids mol.

antigen ~50 kDa

Contient

15 mM sodium azide

species reactivity

mouse, chicken, Chlamydomonas, African green monkey, human, rat, bovine, sea urchin, kangaroo rat

Validation améliorée

independent ( Antibodies)
Learn more about Antibody Enhanced Validation

technique(s)

indirect immunofluorescence: 1:2,000 using cultured human or chicken fibroblasts
radioimmunoassay: suitable
western blot: 1:4,000 using human fibroblast cell extract

Isotype

IgG1

N° d'accession UniProt

application(s)

research pathology

Expédié(e)(s) dans

dry ice

Temp. de stockage

−20°C

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

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Description générale

Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the B-5-1-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tubulin is the major building block of microtubules.
The TUBA4A (tubulin α 4a), is mapped to human chromosome 2q35. The gene codes for a member of the a -tubulin family, and contains 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. The α-subunit of tubulin has molecular weight of 50,000.

Spécificité

Recognizes an epitope located at the C-terminal end of the α-tubulin isoform in a variety of organisms.

Immunogène

Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

Application

Monoclonal Anti-α-Tubulin antibody produced in mouse has been used:
  • In immunofluorescence Analysis
  • In western blotting/ Immunoblotting
  • For immunolabelling cells in electron microscopy

Actions biochimiques/physiologiques

Tubulin α 4a (TUBA4A) mutation is associated with amyotrophic lateral sclerosis (ALS).
α-Tubulin is a key regulator of cytoskeletal proteins. It mediates cellular developmental stages such as proliferation, migration, signalling and also maintains the shape of the cell. α-Tubulin controls trafficking, signaling and cellular tensegrity mediated by microtubules. The encoded protein is associated with the development and progression of cancer. α-Tubulin acetylation potentiates the metastatic property of breast cancer. Mutation in TUBA4A is associated with the development of various types of cancers, such as oral cancer, breast cancer, rectal cancer, lung cancer and prostate cancer. In addition, variation in the TUBA4A leads to sporadic amyotrophic lateral sclerosis (ALS).
Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and the cytoskeleton.
Tubulin α mediates the formation of cytoskeletal protein polymers. It is necessary for cell cycle and controls cellular movement and signaling. Tubulin α is known to maintain the shape of the cells. It is an active part of oncogenesis and progression of different types of cancer. Posttranslationally modified Tubulin α governs microtubule functions including cell trafficking and cellular tensegrity.

Forme physique

Liquid

Stockage et stabilité

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

Certificate of Analysis

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Certificate of Origin

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