Merck
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T8665

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System

peroxidase substrate

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Synonym(s):
TMB
NACRES:
NA.83

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3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA peroxidase substrate

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Niveau de qualité

300

Forme

liquid

Conditions de stockage

protect from light

Expédié(e)(s) dans

wet ice

Temp. de stockage

2-8°C

Application

TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

Attention

This substrate is very light-sensitive and should be protected from direct sunlight or UV sources.

Forme physique

Ready-to-use.

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 3 - STOT SE 1

Code de la classe de stockage

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

129.9 °F - closed cup

Point d'éclair C

54.4 °C - closed cup

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

Certificate of Analysis

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Certificate of Origin

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SDS

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