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U0251

Urease from Canavalia ensiformis (Jack bean)

Type C-3, powder, ≥600,000 units/g solid

Synonyme(s) :

Jack bean urease, Urea amidohydrolase

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A propos de cet article

Numéro CAS:
9002-13-5
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-656-0
NACRES:
NA.54
MDL number:
MFCD00070858
Numéro CE :
3.5.1.5 (BRENDA, IUBMB)

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Nom du produit

Urease from Canavalia ensiformis (Jack bean), Type C-3, powder, ≥600,000 units/g solid

biological source

Canavalia ensiformis

type

Type C-3

form

powder

specific activity

≥600,000 units/g solid

mol wt

~544620 Da

purified by

crystallization

storage temp.

−20°C

Quality Level

200

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Cet article
U400294280666133
Urease from Canavalia ensiformis (Jack bean) Type C-3, powder, ≥600,000 units/g solid

Sigma-Aldrich

U0251

Urease from Canavalia ensiformis (Jack bean)

Urease from Canavalia ensiformis (Jack bean) Type IX, powder, 50,000-100,000 units/g solid

Sigma-Aldrich

U4002

Urease from Canavalia ensiformis (Jack bean)

Urease from Canavalia ensiformis (Jack bean) powder, ~1 U/mg

Sigma-Aldrich

94280

Urease from Canavalia ensiformis (Jack bean)

Urease, Canavalia ensiformis Native urease from Canavalia ensiformis. Catalyzes hydrolysis of urea to carbon dioxide and ammonia. Useful in the determination of urea in body fluids. Note: 1 KU = 1000 units.

Sigma-Aldrich

666133

Urease, Canavalia ensiformis

specific activity

≥600,000 units/g solid

specific activity

50,000-100,000 units/g solid

specific activity

~1 U/mg

specific activity

>150 units/mg material (Nesslers)

biological source

Canavalia ensiformis

biological source

-

biological source

-

biological source

Canavalia ensiformis

form

powder

form

powder

form

powder

form

lyophilized solid

mol wt

~544620 Da

mol wt

~544620 Da

mol wt

Mr ~480000

mol wt

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

purified by

crystallization

purified by

-

purified by

-

purified by

-

Biochem/physiol Actions

Urease aids in the bioavailability of nitrogen in plants. It is implicated in defense mechanism and exhibits insecticidal activity. In addition, urease exhibits antifungal activity by retarding growth and affecting membrane integrity of filamentous fungi and yeasts.
Urease was shown to induce vacuolation in Hela cells, an effect dependent on the ability of urease to convert urea to ammonia. [1]

General description

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Urease is a nickel-dependent metalloenzyme , found in various plants, bacteria, fungi, and algae. It contains two distinct subunits-UreA(26.5 kDa) and UreB (60.3–61.6 kDa).

Other Notes

One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Classe de stockage

11 - Combustible Solids

wgk

WGK 1

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Certificats d'analyse (COA)

Lot/Batch Number
0000490774
0000490772
0000490773
0000490773
0000490772

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

J Jessy et al.
The Biochemical journal, 277 ( Pt 3), 693-696 (1991-08-01)
Recent studies showed that hyperammonaemia caused many of the metabolic changes in portacaval-shunted rats, a model of hepatic encephalopathy. These changes included a depression in the cerebral metabolic rate of glucose (CMRGlc), an indication of decreased brain function. 2. The
Barbara Krajewska et al.
Plant physiology and biochemistry : PPB, 43(7), 651-658 (2005-07-19)
A pH-variation study of jack bean (Canavalia ensiformis) urease steady-state kinetic parameters and of the inhibition constant of boric acid, a urease competitive inhibitor, was performed using both noninhibitory organic (MES, HEPES and CHES) and inhibitory inorganic (phosphate) buffers, in
J Ryan Feathers et al.
Journal of structural biology: X, 5, 100047-100047 (2021-04-06)
The resolution of cryo-EM reconstructions is fundamentally limited by the Nyquist frequency, which is half the sampling frequency of the detector and depends upon the magnification used. In principle, super-resolution imaging should enable reconstructions to surpass the physical Nyquist limit
T L Cover et al.
Infection and immunity, 59(4), 1264-1270 (1991-04-01)
Concentrated broth culture supernatants from 50 to 60% of Helicobacter pylori strains induce eukaryotic cell vacuolation in vitro. A quantitative assay for cell vacuolation was developed on the basis of the rapid uptake of visibly vacuolated HeLa cells was significantly
Anne H S Martinelli et al.
Biochimica et biophysica acta, 1840(3), 935-944 (2013-11-19)
Ureases are metalloenzymes involved in defense mechanisms in plants. The insecticidal activity of Canavalia ensiformis (jack bean) ureases relies partially on an internal 10kDa peptide generated by enzymatic hydrolysis of the protein within susceptible insects. A recombinant version of this
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