U7752

Urease from Canavalia ensiformis (Jack bean)

Name: Urease from <I>Canavalia ensiformis</I> (Jack bean)
Brand: SIGMA
Price: 103 EUR

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A propos de cet article

Numéro CAS:

9002-13-5

UNSPSC Code:

12352204

eCl@ss:

32160410

EC Number:

232-656-0

NACRES:

NA.54

MDL number:

MFCD00070858

Numéro CE :

3.5.1.5 (BRENDA, IUBMB)

Principaux documents

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biological source

Canavalia ensiformis

form

solid

mol wt

hexamer 545 kDa
trimer ~272 kDa

contains

~20% dithiothreitol

suitability

suitable for marker for electrophoresis (non-denaturing PAGE)

storage temp.

−20°C

Quality Level

200

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Cet article	U0251	U1500	U4002
Sigma-Aldrich U7752 Urease from Canavalia ensiformis (Jack bean)	Sigma-Aldrich U0251 Urease from Canavalia ensiformis (Jack bean)	Sigma-Aldrich U1500 Urease from Canavalia ensiformis (Jack bean)	Sigma-Aldrich U4002 Urease from Canavalia ensiformis (Jack bean)
biological source Canavalia ensiformis	biological source Canavalia ensiformis	biological source Canavalia ensiformis	biological source -
form solid	form powder	form powder	form powder
suitability suitable for marker for electrophoresis (non-denaturing PAGE)	suitability -	suitability -	suitability -
mol wt hexamer 545 kDa, trimer ~272 kDa	mol wt ~544620 Da	mol wt ~544620 Da	mol wt ~544620 Da
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C
Quality Level 200	Quality Level 200	Quality Level 300	Quality Level 200

Application

Urease from Canavalia ensiformis (Jack bean) has been used:

as a component of the molecular mass standard in electrophoresis[1]
as a component of the standard in size-exclusion chromatography[2]
as a reference in native-polyacrylamide gel electrophoresis (PAGE)[3]

Biochem/physiol Actions

Urease is an essential enzyme of the nitrogen cycle.[4] It plays a role in degrading urea into ammonia and carbamate.[4][5] Urease provides a source of nitrogen for growth to various organisms. It also plays a major role in seed germination and seed chemical defense.[6]

General description

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620

Urease is a multi-subunit nickel-dependent metalloenzyme. It is found abundantly in fungi, yeast, plants, and bacteria.[4] It belongs to the superfamily of amidohydrolases and phosphotriesterases.[5] Urease is also found in leguminous seeds.[6]

Other Notes

Contains approx. 1 mg protein

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Classe de stockage

11 - Combustible Solids

wgk

WGK 1

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Ureases: Historical aspects, catalytic, and non-catalytic properties - A review.

Karine Kappaun et al.

Journal of advanced research, 13, 3-17 (2018-08-11)

Urease (urea amidohydrolase, EC 3.5.1.5) is a nickel-containing enzyme produced by plants, fungi, and bacteria that catalyzes the hydrolysis of urea into ammonia and carbamate. Urease is of historical importance in Biochemistry as it was the first enzyme ever to

Enhanced catalytic properties of novel (alphagamma) 2 heterohexameric Rhodobacter capsulatus xanthine dehydrogenase by separate expression of the redox domains in Escherichia coli

Wang C, et al.

Biochemical Engineering Journal, 119, 1-8 (2017)

The RESP AI model accelerates the identification of tight-binding antibodies.

Jonathan Parkinson et al.

Nature communications, 14(1), 454-454 (2023-01-29)

High-affinity antibodies are often identified through directed evolution, which may require many iterations of mutagenesis and selection to find an optimal candidate. Deep learning techniques hold the potential to accelerate this process but the existing methods cannot provide the confidence

Soil urease inhibition by various plant extracts.

Muhammad Ajmal Rana et al.

PloS one, 16(10), e0258568-e0258568 (2021-10-15)

Urea is the most popular and widely used nitrogenous fertilizer. High soil urease activity rapidly hydrolyses applied urea to ammonia which contributes to soil nitrogen (N) losses and reduces N use efficiency of crop plants. The ammonia losses can be

Crystal structure of the first plant urease from jack bean: 83 years of journey from its first crystal to molecular structure.

Anuradha Balasubramanian et al.

Journal of molecular biology, 400(3), 274-283 (2010-05-18)

Urease, a nickel-dependent metalloenzyme, is synthesized by plants, some bacteria, and fungi. It catalyzes the hydrolysis of urea into ammonia and carbon dioxide. Although the amino acid sequences of plant and bacterial ureases are closely related, some biological activities differ

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Product Information Sheet - U7752

Instructions

Questions

il y a 10 mois

Do you know the activity in U/mg of this urease preparation?

1 réponse
1. Technical Support
  
  il y a 9 mois
  
  This product is intended for use as a molecular weight marker in electrophoresis. The activity is not determined. Please see the link below to review other Urease products that offer a unit activity:
  https://www.sigmaaldrich.com/substance/ureasefromcanavaliaensiformisjackbean123459002135
  
  Utile ?

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