Merck
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V9264

Sigma-Aldrich

Anti-Vinculin antibody, Mouse monoclonal

clone hVIN-1, purified from hybridoma cell culture

Synonym(s):
Monoclonal Anti-Vinculin antibody produced in mouse
Numéro MDL:
NACRES:
NA.41

Source biologique

mouse

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

antibody product type

primary antibodies

Clone

hVIN-1, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen 116 kDa

species reactivity

frog, chicken, mouse, canine, human, bovine, rat, turkey

Conditionnement

antibody small pack of 25 μL

Concentration

~1.0 mg/mL

technique(s)

immunocytochemistry: 5-10 μg/mL using HS-68 human fibroblast cell culture
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 0.05-0.1 μg/mL using HS-68 total cell extract

Isotype

IgG1

N° d'accession UniProt

application(s)

research pathology

Expédié(e)(s) dans

dry ice

Temp. de stockage

−20°C

Gene Information

human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)

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Description générale

Anti-Vinculin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hVIN-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with vinculin purified from human uterus. Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions.[1] In muscle, vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletal muscle), neuromuscular junctions, and the membrane associated dense bodies of smooth muscle.

Spécificité

Specifically labels vinculin at cell-cell and cell-substrate contacts. Reacts strongly with human vinculin. Shows cross-reactivity with smooth muscle metavinculin.

Immunogène

purified human vinculin from uterus.

Application

Anti-Vinculin antibody, Mouse monoclonal has been used in
  • immunoblotting[2][3]
  • immunofluorescence staining[4][5]
  • immunocytochemistry
  • immunohistochemistry and enzyme linked immunosorbent assay (ELISA).

Actions biochimiques/physiologiques

Vinculin functions as one of several interacting proteins involved in anchoring F-actin to the membrane.[1] It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes.[6]

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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