Anti-Ki67 Antibody, clone 1O15, ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

Antigen KI-67, Proliferation marker protein Ki-67, Antigen Ki67, Antigen identified by monoclonal antibody Ki-67

Source biologique

rabbit (recombinant)


expressed in HEK 293 cells

Forme d'anticorps

purified antibody

antibody product type

primary antibodies


1O15, monoclonal
recombinant monoclonal

Gamme de produits

ZooMAb® learn more



Poids mol.

calculated mol wt 358.69 kDa
observed mol wt ~350 kDa

species reactivity



antibody small pack of 25 μL

Caractéristiques du produit alternatif plus écologique

Waste Prevention
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Design for Energy Efficiency
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Validation améliorée

recombinant expression
Learn more about Antibody Enhanced Validation


immunocytochemistry: suitable using 1:100
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:400
western blot: suitable using 1:1,000





N° d'accession UniProt

Expédié(e)(s) dans


Temp. de stockage


Gene Information

human ... P46013(4288)

Description générale

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb® here.


Clone 1O15 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Proliferation marker protein Ki-67. It targets an epitope with in 14 amino acids from the C-terminal half.


KLH conjugated linear peptide corresponding to 14 amino acids from the C-terminal half of human Proliferation marker protein Ki-67.


Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Ki67 in HUVEC, HeLa, and A431 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:400 dilution from a representative lot detected Ki67 in human tonsil and human skin tissue sections.
Anti-Ki67, clone 1O15 ZooMAb, Cat. No ZRB1007, is a recombinant Rabbit Monoclonal Antibody that specifically targets Proliferation marker protein Ki-67 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Description de la cible

Proliferation marker protein Ki-67 (UniProt: P46013; also known as Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67) is encoded by the MKI67 gene (Gene ID: 4288) in human. Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle. Its expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen is undetectable. Ki67 is hyperphosphorylated in mitosis. Ki67 is required to maintain individual mitotic chromosomes and it disperses in the cytoplasm following nuclear envelope disassembly. It is shown to associate with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. It prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier. Two isoforms of Ki67 have been described that are produced by alternative splicing. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.


120 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


11 - Combustible Solids

WGK Allemagne


Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

Certificat d'analyse

Certificat d'origine

Zhi Teng et al.
Oncology reports, 43(2), 503-515 (2020-01-03)
Acute lymphoblastic leukemia (ALL) is a malignant hematological disease. Tanshinone IIA (Tan IIA) has antitumor activity in vitro and in vivo. The aim of the present study was to investigate the effects of Tan IIA in combination with imatinib (IM) on the proliferation, apoptosis, migration...

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