ZRB1175

Sigma-Aldrich

Anti-p53 Antibody, clone 6O23, ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

Source biologique

rabbit (recombinant)

Recombinant

expressed in HEK 293 cells

Forme d'anticorps

purified antibody

antibody product type

primary antibodies

Clone

6O23, recombinant monoclonal

Gamme de produits

ZooMAb® learn more

Forme

lyophilized

Poids mol.

calculated mol wt 43.65 kDa
observed mol wt ~53 kDa

species reactivity

human

species reactivity (predicted by homology)

bovine, rhesus macaque

Conditionnement

antibody small pack of 25 μL

Caractéristiques du produit alternatif plus écologique

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

Validation améliorée

recombinant expression
Learn more about Antibody Enhanced Validation

Application(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable (peptide)

Isotype

IgG

Conjugué

unconjugated

Autre catégorie plus écologique

Aligned

Expédié(e)(s) dans

ambient

Temp. de stockage

2-8°C

Description générale

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Spécificité

Clone 6O23 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects p53 protein. It targets an epitope within 12 amino acids from the N-terminal region.

Immunogène

KLH-conjugated linear peptide corresponding to 12 amino acids from the N-terminal region of human p53.

Application

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected p53 in HEK293 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:10-100 dilution from a representative lot detected p53 in human ovarian cancer and human colon cancer tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected p53 in A431 and HaCaT cell lines.

Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.
Anti-p53, clone 6O23 ZooMAb , Cat. No. ZRB1175, is a highly specific recombinant rabbit monoclonal antibody that detects p53 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Description de la cible

Cellular tumor antigen p53 (UniProt: P04637; also known as Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53) is encoded by the TP53 (also known as p53) gene (Gene ID: 7157) in human. p53, a well-conserved phosphoprotein, is one of the best-known tumor suppressors. Human p53 consists of 393 amino acids assembled into five structurally and functionally different domains. It acts as a sequence-specific nuclear transcription factor that binds to defined consensus sites within DNA as a tetramer and represses transcription of a set of genes involved in cell growth stimulation, while activating a different set of genes involved in cell cycle control. It causes growth arrest before either DNA replication thereby providing a window for DNA repair or elimination of cells with severely damaged DNA strands. In some cell types, however, p53 activation results in apoptosis as a means of eliminating severely damaged cells. In normal, non-activated cells, the p53 signaling network is not active. However, p53 signaling is activated in cells as a response to various signals. Carcinogen-induced DNA damage, abnormal proliferative signals, hypoxia, and loss of cell adhesion are some of the most common signals that activate p53. The final outcome of p53 activation depends on many factors and is mediated largely through the action of downstream effector genes transactivated by p53. In unstressed cells, p53 is latent and is maintained at low levels by targeted, ubiquitin-mediated degradation mediated by MDM2 and many other ubiquitin ligases. MDM2 functions as an E3 ligase to ubiquitinate p53 and force its export from the nucleus to the cytoplasm, where p53 is degraded by the proteasome. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Allemagne

WGK 1

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

Certificat d'analyse
Certificat d'origine

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