ZRB1306

Sigma-Aldrich

Anti-HNF-1β Antibody, clone 1M22 ZooMAb® Rabbit Monoclonal

newenhanced validation

recombinant, expressed in HEK 293 cells

Niveau de qualité

200

Source biologique

rabbit

Recombinant

expressed in HEK 293 cells

Forme d'anticorps

purified antibody

antibody product type

primary antibodies

Clone

1M22, recombinant monoclonal

Description

recombinant, expressed in HEK 293 cells

Gamme de produits

ZooMAb® learn more

Forme

lyophilized

Poids mol.

calculated mol wt 61.32 kDa
observed mol wt ~64 kDa

species reactivity

human

Conditionnement

antibody small pack of 25 μL

Validation améliorée

recombinant expression
Learn more about Antibody Enhanced Validation

Application(s)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

Isotype

IgG

Séquence de l'épitope

C-terminal half

Conjugué

unconjugated

N° d'accession Protein ID

N° d'accession UniProt

Expédié(e)(s) dans

ambient

Temp. de stockage

2-8°C

Description générale

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Spécificité

Clone 1M22 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Hepatocyte nuclear factor 1β . It targets an epitope within 24 amino acids from the C-terminal half.

Immunogène

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal half of human Hepatocyte nuclear factor 1-beta.

Application

Quality Control Testing

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected HNF-1β in A549 cell lysate.

Tested applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected HNF-1β in HepG2 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected HNF-1 β in human kidney tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound HNF-1β with a KD of 2.7 x 10-9 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-HNF-1β, clone 1M22 ZooMAb®, Cat. No. ZRB1306, is a recombinant Rabbit monoclonal antibody that specifically targets HNF-1b and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Description de la cible

Hepatocyte nuclear factor 1β (UniProt: P35680; also known as HNF-1-beta, HNF-1B, Homeoprotein LFB3, Transcription factor 2, TCF-2, Variant hepatic nuclear factor 1, vHNF1) is encoded by the HNF1B (also known as TCF2) gene (Gene ID: 6928) in human. HNF-1β is a transcription factor of the HNF1 homeobox family that binds to the inverted palindrome 5′-GTTAATNATTAAC-3′. It binds to DNA as a dimer. It controls endoderm development and is shown to be crucial for pancreatic and renal development. It is also involved in the hepatobiliary specification of hepatoblasts to cholangiocytes during liver development and is strongly expressed throughout adult biliary epithelium. Over-expression of HNF-1β is associated with pathological subtype of primary tumor and its expression in hepatocellular carcinoma is associated with the change of phenotype on recurrence. Mutations in HNF1B are known to cause Renal cysts and diabetes syndrome (RCAD), an autosomal dominant disorder comprising non-diabetic disease resulting from abnormal renal development. HNF1B knockout mice are shown to suffer from severe neonatal cholestasis and jaundice due to the abnormally developed gall bladder and dysfunctional intrahepatic bile ducts. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kolatsi-Joannou, M., et al. (2001). J. Am. Soc. Nephrol. 12(10); 2175-2180; Yu, D-D., et al. (2015). Sci. Rep. 5; Article 13319).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

11 - Combustible Solids

WGK Allemagne

WGK 1

Certificat d'analyse

Certificat d'origine

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