Millex® Syringe Filters

Sterile and nonsterile Millex® syringe filters for sample preparation

Setting the standard in small-volume filtration (1-200 mL), the unsurpassed consistency of Millex® syringe filters in sample preparation has led to the development of many methods specifying their use.

Featuring reduced sample loss due to minimal hold-up volumes, Millex® syringe filters are ideal for use in a variety of applications including:

  • HPLC sample prep
  • Filtration of antibiotics and tissue culture additives
  • Dissolution testing

Our controlled manufacturing process ensures reliable filter performance and our sterile devices include a certificate of quality. Available with many different membrane and housing options, Millex® syringe filters are compatible with a variety of solvents and sample types.

Membrane types include:

  • Durapore® PVDF membrane: Recommended for low protein binding
  • Millipore Express® PLUS polyethersulfone membrane: Recommended for fast flow and low binding for cell culture media preparation
  • Hydrophilic PTFE membrane: Recommended for low HPLC-UV extractables

Recommended diameter by sample volume:

  • <1 mL: 4 mm
  • 1-10 mL: 13 mm
  • 10-100 mL: 25 mm, 33 mm

Sterile Millex® Syringe Filters

  • Applications include sterilizing cell culture reagents such as cell culture media, antibiotics, supplements, sera, viruses for infection, and other biological solutions
  • Fast flow and low binding for cell culture media preparation with Millipore Express® PLUS polyethersulfone (PES) membrane
  • Durapore® PVDF membrane is the lowest binding membrane for protein-rich solutions
  • Many are CE-marked and/or approved as medical devices, meaning that they can be used to filter pharmaceutical solutions
  • Minimal leaching of impurities into filtered solutions
  • 50mm Millex® syringe filters have hydrophobic chemistry for gas filtration and in-line vacuum pump protection

Nonsterile Millex® Syringe Filters

  • Applications include filtration-based sample preparation for dissolution testing, HPLC, UHPLC, LC-MS, ion chromatography (IC), and general particulate removal
  • Hydrophilic PTFE Millex® filters are HPLC-certified for low extractables
  • Best recovery of protein samples with hydrophilic Durapore® PVDF Millex® syringe filters
  • Automation-compatible, non-sterile Millex® syringe filter units have a domed housing which enables smooth, reliable delivery in automated sample preparation workstations by eliminating shingling between filters on the transport rack.
  • Non-sterile HPF Millex® filters include a graduated glass fiber prefilter to removed larger particles and either a 0.20 µm or 0.45 µm membrane filter for fine filtration. This combination of membranes provides significantly greater throughput than standard filters without prefiltration media, especially when filtering particle laden solutions.
  • 50mm Millex® syringe filters have hydrophobic chemistry for gas filtration and in-line vacuum pump protection

Learn more about factors affecting analyte binding to syringe filters.


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