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Quality Control Ranges: Milliplex® Human Adipokine Magnetic Bead Panel 2
I HADK2-109 4689 - 9739 pg/mL
Control II HADK2-209 21943 - 45574 pg/mL
IL-8 Control I HADK2-109 70 - 145 pg/mL
Control II HADK2-209 302 - 626 pg/mL
MCP-1 Control
The Effective Use of Protein Kinase Inhibitors
77 28 29 63 104 91 9 92 42 94 111 94 70 22 72 104 101 84
87 110 29 12 12 49 66 28 94 79 20 117 64 43 70 93 71 22 90 87 74
The Effective Use of Protein Kinase Inhibitors
77 28 29 63 104 91 9 92 42 94 111 94 70 22 72 104 101 84
87 110 29 12 12 49 66 28 94 79 20 117 64 43 70 93 71 22 90 87 74
Data Sheet - SRP5063
0.1 mM
PMSF, and 25% glycerol.
Molecular mass: ∼79 kDa
Purity: 70–95% (SDS-PAGE, see Figure 1)
Specific Activity: 81–109 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product
Improved Method for Adenovirus and Lentivirus Purification using the Fast-Trap™ Virus Purification and Concentration Kit
Gradient
Amount crude
adenovirus (ivp)
5.81 x 109 5.81 x 109
Amount purified
adenovirus (ivp)
4.05 x 109 2.64 x 109
% virus recovery 70 45
Table 1.
Adenovirus purification
results using
User Guide-RAB1021
107
Cell Culture Supernatants 90 70-109
Linearity
Sample Type Serum Plasma Cell Culture Supernatants
1:2
Average % of Expected 103 104 103
Range (%) 98-109 101-107 97-107
1:4
Average % of
Data Sheet - P3326
1989).
5. Giese, N.A., et al., Science, 236, 1315 (1987).
6. Ross, R., et al., Cell, 46, 155 (1986).
7. Pierce, G.F., et al., J. Cell Biol., 109, 429 (1989).
8. Ross, R., et al., Science, 248, 1009 (1990
ZIC-HILIC Separation of organophosphate nerve agent metabolites
. 150454)
Injection: 2 µL samples diluted in 70:30 acetonitrile/water v/v
Detection: LC-MS in negative mode
TIC from SIM m/z: 179 (+ fragment 109, 123, 137, 151, 191, 247 and 286)
Flow Rate
MILLIPLEX®MAP Human Myokine Panel is an optimized quantitative immunoassay that simultaneously measures 15 novel muscle-secreted factors
15 Myostatin FABP3 Irisin FSTL1 OSM IL-6 FGF21 Musclin
1:4 dil 111 85 58 105 100 97 95 74 92 106 73 106 77 91 109
1:8 dil 123 84 48 108 98 91 94 63 87 105
Product Information Sheet - CYP1
1. Pellet yeast cells (2 x 109 cells) from mid-log phase
(1-5 x 107 cells/ml) by centrifugation at
3,000−5,000 x g for 5 minutes.
2. Resuspend the pellet with 4−6 volumes of distilled
water (5
VirusExpress® 293 AAV Production Platform
supply chain concerns
• Proven process performance at 3 L scale with
genome titer exceeding 8 x 109 gc/mL
• Comprehensive user protocols to guide from
seed train through at-scale transfection and
Data Sheet - C8195
on dry ice and storage at –70 C is
recommended. Repeated freezing and thawing is not
recommended.
References
1. Kidmose, R.T. et al., Proc. Nat. Acad. Sci. USA,
109(38), 15425-15430 (2012).
Comparison of Chromogenic Coliform Agar Membrane Filter Sets For Water Filtration Applications
productivity rates are listed in Table 6.
Table 6. Performance criteria and productivity control strains according to ISO 9308-1:2014 (table 1)
The recovery rate of 70 % is equivalent to a productivity
Neutralizing Efficiency of Lecithin and Polysorbate 80 in ICR Contact Plates and Swabs for Surface Monitoring
pl
es
SCDB w/o neutralizer
102
89
104
111
85
97
80
109
99
84
91
117
101
108
99
109
99
128
87
108
83
92 95 93
TB007 Bacteriophage CE6
concentration of cells should be about 5 x 108/ml.
4. Add the λCE6 stock to a final concentration of 2–4 x 109 pfu/ml. Incubate 5 min without shaking.
Note: The multiplicity of infection (MOI) should be between
Monitoring low dose API blend uniformity with Parteck® M
content obtained by primary method vs
model predicted values.
0 1 2 3 6 7 84 9 10 11
130
90
100
110
120
80
70
D
ru
g
co
Optimization of Bacterial Culture and Plasmid Purification with Eppendorf Conical Tubes 25 mL
15ml tube
Pl
as
m
id
D
N
A
Yi
el
d
ug
1.6 x 1010
1.2 x 1010
8 x 109
4 x 109
15 mL culture
25 mL tube
Suspension MDCK Cell Culture in EX-CELL MDCK Serum-Free Medium
remain susceptible to viral infection, and
in this study, Canine Adenovirus (CAV) titers in excess of 109
infectious particles/mL were obtained.
Materials
Cells and Virus
• Madin Darby Canine Kidney
Product Information Sheet - M2378
942
(1996).
2. Schömig, V.J. et al., RSC Adv., 6, 44932-44943
(2016).
3. Libao-Mercado, A.J., and de Lange, C.F.M.,
Livestock Sci., 109(1-3), 141-144 (2007).
4. Perez-Vilar, J., and Hill
Product Information Sheet - M1778
942
(1996).
2. Schömig, V.J. et al., RSC Adv., 6, 44932-44943
(2016).
3. Libao-Mercado, A.J., and de Lange, C.F.M.,
Livestock Sci., 109(1-3), 141-144 (2007).
4. Perez-Vilar, J., and Hill
Data Sheet - K4761
recombinant human TRAIL solution should be
stored in aliquots at −70 °C. Avoid multiple freeze-thaw
cycles. It is stable for at least 6 months at this
temperature.
Product Profile
Recombinant human
Anti-Brain-derived Neurotrophic Factor (B9186)
at −20 °C or −70 °C. Reconstituted
product may be stored at 2-8 °C for up to one month.
For prolonged storage, the product may be frozen in
working aliquots at −20 °C to −70 °C for 6 months
without
Data Sheet - O8762
molecular mass of the
monomer is 109 kDa, but as a result of glycosylation,
the recombinant monomer migrates at >150 kDa on
reducing SDS-PAGE.1,2
Oncostatin M is a member of the IL-6-type family of
cytokines
Data Sheet - S7901
Interaction, New York: Springer-Verlag,
p. 206 (1979).
8. Orr-Urtreger, A., et al., Development, 109,
911(1990).
9. Manova, K., et al., Development, 110, 1057 (1990).
10. Arakawa, T., et al., J.
Product Information Sheet-03143422001
mg 10 109 142 001
100 mg 10 109 169 001
1 2 3
1 2 3
https://www.sigmaaldrich.com
Pa
pe
r I
de
nt
C
od
e
MOUSE ANTI-NERVE GROWTH FACTOR RECEPTOR MONOCLONAL ANTIBODY
up to 12 months after date of receipt. After
reconstitution maintain at -20°C to -70°C in undiluted aliquots for up to 6 months. Avoid
repeated freeze/thaw cycles. Glycerol (ASC grade or better) can be
Data Sheet - U3133
toward various aglycones
using uridine diphospho[U-14C] glucuronic acid.
Anal. Biochem., 109, 321-329 (1980).
6. Strassburg, C. P., et al., Expression of the UDP-
glucuronosyltransferase 1A Locus in
User Guide-RAB1317
follows:
Sample Type Average % Recovery Range (%)
Serum 113 109-116
Plasma 113 98-147
Cell Culture Supernatants 102 95-109
Linearity
Sample Type Serum Plasma Cell Culture Supernatants
User Guide-RAB0097
Plasma 122 109-142
Cell Culture Supernatants 112 104-120
Linearity
Sample Type Serum Plasma Cell Culture Supernatants
1:2
Average % of Expected 82 102 101
Range (%) 70-94 92-120 93-109
1:4
CSK Active (C1620)
CSK
40
72
100
130
170
55
33
25
Figure 2.
Specific Activity of Lot Number 019K0641:
109 nmole/min/mg
0
150,000
300,000
450,000
600,000
0 50 100 150 200
Protein (ng)
A
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