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Detection and Quantification of MicroRNAs by Ligase-Assisted Sandwich Hybridization on a Microarray
Iizuka R, et al.
Microarrays, 1368 (2016)
Graphene oxide size and oxidation degree govern its supramolecular interactions with siRNA
Reina G, et al.
Nanoscale, 10(30), 5965-5974 (2018)
Giacomo Reina et al.
Nanoscale, 10(13), 5965-5974 (2018-03-16)
Several studies have demonstrated the ability of graphene oxide (GO) to efficiently adsorb small-interfering RNA (siRNA) on its surface and to transport it into cells. However, studies on whether and how siRNA interacts with GO are still inconclusive. In this...
Whole mount RNA-FISH on ovules and developing seeds
Bleckmann A and Dresselhaus T
Chemosphere, 159-171 (2017)
Real-time assay for testing components of protein synthesis
Rosenblum G, et al.
Nucleic Acids Research, 40(12), e88-e88 (2012)
Ryo Iizuka et al.
Methods in molecular biology (Clifton, N.J.), 1368, 53-65 (2015-11-29)
Extracellular microRNAs (miRNAs) in body fluids have been identified as promising biomarkers for different human diseases. The high-throughput, multiplexed detection and quantification of these miRNAs are highly beneficial for the rapid and accurate diagnosis of diseases. Here, we developed a...
Gabriel Rosenblum et al.
Nucleic acids research, 40(12), e88-e88 (2012-03-17)
We present a flexible, real-time-coupled transcription-translation assay that involves the continuous monitoring of fluorescent Emerald GFP formation. Along with numerical simulation of a reaction kinetics model, the assay permits quantitative estimation of the effects on full-length protein synthesis of various...
Andrea Bleckmann et al.
Methods in molecular biology (Clifton, N.J.), 1669, 159-171 (2017-09-25)
A key element to understand developmental and reproductive processes like germline development, double fertilization, and embryogenesis is the study of cell-specific gene expression patterns which is best analyzed by RNA in situ hybridization. Different visualization techniques have been established to...
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