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Detection and Quantification of MicroRNAs by Ligase-Assisted Sandwich Hybridization on a Microarray
Iizuka R, et al.
Microarrays, 1368 (2016)
Graphene oxide size and oxidation degree govern its supramolecular interactions with siRNA
Reina G, et al.
Nanoscale, 10(30), 5965-5974 (2018)
Giacomo Reina et al.
Nanoscale, 10(13), 5965-5974 (2018-03-16)
Several studies have demonstrated the ability of graphene oxide (GO) to efficiently adsorb small-interfering RNA (siRNA) on its surface and to transport it into cells. However, studies on whether and how siRNA interacts with GO are still inconclusive. In this
Real-time assay for testing components of protein synthesis
Rosenblum G, et al.
Nucleic Acids Research, 40(12), e88-e88 (2012)
Andrea Bleckmann et al.
Methods in molecular biology (Clifton, N.J.), 1669, 159-171 (2017-09-25)
A key element to understand developmental and reproductive processes like germline development, double fertilization, and embryogenesis is the study of cell-specific gene expression patterns which is best analyzed by RNA in situ hybridization. Different visualization techniques have been established to
Gabriel Rosenblum et al.
Nucleic acids research, 40(12), e88-e88 (2012-03-17)
We present a flexible, real-time-coupled transcription-translation assay that involves the continuous monitoring of fluorescent Emerald GFP formation. Along with numerical simulation of a reaction kinetics model, the assay permits quantitative estimation of the effects on full-length protein synthesis of various
Whole mount RNA-FISH on ovules and developing seeds
Bleckmann A and Dresselhaus T
Chemosphere, 159-171 (2017)
Ryo Iizuka et al.
Methods in molecular biology (Clifton, N.J.), 1368, 53-65 (2015-11-29)
Extracellular microRNAs (miRNAs) in body fluids have been identified as promising biomarkers for different human diseases. The high-throughput, multiplexed detection and quantification of these miRNAs are highly beneficial for the rapid and accurate diagnosis of diseases. Here, we developed a
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