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Francesca De Faveri et al.
Autophagy, 16(7), 1314-1331 (2019-10-28)
Activation of trypsinogen (formation of trypsin) inside the pancreas is an early pathological event in the development of acute pancreatitis. In our previous studies we identified the activation of trypsinogen within endocytic vacuoles (EVs), cellular organelles that appear in pancreatic
Lydia R Heasley et al.
Molecular biology of the cell, 27(3), 442-450 (2015-12-19)
The highly conserved family of septin proteins has important functions in cytokinesis in mitotically proliferating cells. A different form of cytokinesis occurs during gametogenesis in Saccharomyces cerevisiae, in which four haploid meiotic products become encased by prospore membrane (PSMs) and
Ki-Hee Song et al.
Light, science & applications, 9, 92-92 (2020-06-09)
Spectroscopic single-molecule localization microscopy (sSMLM) was used to achieve simultaneous imaging and spectral analysis of single molecules for the first time. Current sSMLM fundamentally suffers from a reduced photon budget because the photons from individual stochastic emissions are divided into
Samir Rahman et al.
Nucleic acids research, 45(6), 3017-3030 (2016-12-10)
Enhancers are intergenic DNA elements that regulate the transcription of target genes in response to signaling pathways by interacting with promoters over large genomic distances. Recent studies have revealed that enhancers are bi-directionally transcribed into enhancer RNAs (eRNAs). Using single-molecule
Raghav Garg et al.
ACS nano, 11(6), 6301-6311 (2017-05-27)
Graphene, a honeycomb sp
Aaron G Wexler et al.
Cell reports, 36(10), 109683-109683 (2021-09-09)
Clostridioides difficile is the leading cause of nosocomial intestinal infections in the United States. Ingested C. difficile spores encounter host bile acids and other cues that are necessary for germinating into toxin-producing vegetative cells. While gut microbiota disruption (often by antibiotics)
António J Pereira et al.
Methods in molecular biology (Clifton, N.J.), 545, 145-164 (2009-05-29)
Progress from our present understanding of the mechanisms behind mitosis has been compromised by the fact that model systems that were ideal for molecular and genetic studies (such as yeasts, C. elegans, or Drosophila) were not suitable for intracellular micromanipulation.
Rajendra K Gangalum et al.
Investigative ophthalmology & visual science, 50(7), 3283-3290 (2009-02-17)
All crystallins have non-crystallin catalytic functions. Because catalytic functions do not require large concentrations of protein, as are seen in the lens, there is a perception of dichotomy in the catalytic/physiological function of crystallins within and outside the lens. The
Grace E Peng et al.
Nature chemical biology, 17(5), 558-566 (2021-03-03)
G-protein-coupled receptor-regulated cAMP production from endosomes can specify signaling to the nucleus by moving the source of cAMP without changing its overall amount. How this is possible remains unknown because cAMP gradients dissipate over the nanoscale, whereas endosomes typically localize
Sergi Aranda et al.
Molecular and cellular biology, 28(19), 5899-5911 (2008-08-06)
Raf-MEK-extracellular signal-regulated kinase (Erk) signaling initiated by growth factor-engaged receptor tyrosine kinases (RTKs) is modulated by an intricate network of positive and negative feedback loops which determine the specificity and spatiotemporal characteristics of the intracellular signal. Well-known antagonists of RTK
Shella Saint Fleur-Lominy et al.
Cell reports, 24(11), 3045-3060 (2018-09-13)
T cell acute lymphoblastic leukemia (T-ALL) is commonly associated with activating mutations in the NOTCH1 pathway. Recent reports have shown a link between NOTCH1 signaling and intracellular Ca2+ homeostasis in T-ALL. Here, we investigate the role of store-operated Ca2+ entry
Ronja Göhde et al.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 376(1821), 20190759-20190759 (2021-02-09)
Neurosecretory vesicles are highly specialized trafficking organelles that store neurotransmitters that are released at presynaptic nerve endings and are, therefore, important for animal cell-cell signalling. Despite considerable anatomical and functional diversity of neurons in animals, the protein composition of neurosecretory
Vincent Gaggioli et al.
PLoS genetics, 16(12), e1008948-e1008948 (2020-12-16)
During metazoan development, the cell cycle is remodelled to coordinate proliferation with differentiation. Developmental cues cause dramatic changes in the number and timing of replication initiation events, but the mechanisms and physiological importance of such changes are poorly understood. Cyclin-dependent
Sebastian Sorge et al.
Cell reports, 31(7), 107659-107659 (2020-05-21)
The mitochondrial electron transport chain (ETC) enables essential metabolic reactions; nonetheless, the cellular responses to defects in mitochondria and the modulation of signaling pathway outputs are not understood. We show that Notch signaling and ETC attenuation via knockdown of COX7a
Fanghai Liu et al.
Biochemical and biophysical research communications, 527(1), 194-199 (2020-05-25)
Peritrichously flagellated bacteria such as Escherichia coli (E. coli) perform chemotaxis by a biased random walk toward various chemicals, which was driven by the bacterial flagellar motor. Fructose, a typical monosaccharide that can attract E. coli. However, little is known about the
Annalisa Calò et al.
Scientific reports, 10(1), 15664-15664 (2020-09-26)
Changes in the elastic properties of living tissues during normal development and in pathological processes are often due to modifications of the collagen component of the extracellular matrix at various length scales. Force volume AFM can precisely capture the mechanical
Ilaria Zamparo et al.
Cell reports, 29(13), 4334-4348 (2019-12-26)
In mammals, odorant receptors not only detect odors but also define the target in the olfactory bulb, where sensory neurons project to give rise to the sensory map. The odorant receptor is expressed at the cilia, where it binds odorants
Diane Murera et al.
Nanoscale, 11(21), 10493-10503 (2019-05-22)
Carbon-based nanomaterials represent a new tool in future medical applications. Thus, focusing on the evaluation of the degree of their safety has been growing in the last years. In this study we were particularly interested in understanding the impact of
Tiago D Serafim et al.
Nature microbiology, 3(5), 548-555 (2018-03-21)
Sand flies, similar to most vectors, take multiple blood meals during their lifetime1-4. The effect of subsequent blood meals on pathogens developing in the vector and their impact on disease transmission have never been examined. Here, we show that ingestion
Pradeep Kumar Kopparapu et al.
International journal of molecular sciences, 22(13) (2021-07-21)
Staphylococcal aureus (S. aureus), a Gram-positive bacteria, is known to cause various infections. Extracellular vesicles (EVs) are a heterogeneous array of membranous structures secreted by cells from all three domains of life, i.e., eukaryotes, bacteria, and archaea. Bacterial EVs are
Poney Chiang et al.
Microbiology (Reading, England), 154(Pt 1), 114-126 (2008-01-05)
Type IV pili are retractable protein fibres used by many bacterial pathogens for adherence, twitching motility, biofilm development and host colonization. In Pseudomonas aeruginosa, PilB and PilT are bipolar proteins belonging to the secretion NTPase superfamily, and power pilus extension
Mari Sepp et al.
PloS one, 6(7), e22138-e22138 (2011-07-27)
Transcription factor 4 (TCF4 alias ITF2, E2-2, ME2 or SEF2) is a ubiquitous class A basic helix-loop-helix protein that binds to E-box DNA sequences (CANNTG). While involved in the development and functioning of many different cell types, recent studies point
Xuan Li et al.
Cell death discovery, 7(1), 338-338 (2021-11-10)
Necroptosis, a form of programmed cell death, accounts for many inflammations in a wide range of diseases. Diet-induced obesity is manifested by low-grade inflammation in the mediobasal hypothalamus (MBH), and microglia are implicated as critical responsive components for this process.
Gergő Gulyás et al.
Journal of cell science, 133(6) (2020-02-12)
Oxysterol-binding protein (OSBP)-related proteins (ORPs) mediate non-vesicular lipid transfer between intracellular membranes. Phosphoinositide (PI) gradients play important roles in the ability of OSBP and some ORPs to transfer cholesterol and phosphatidylserine between the endoplasmic reticulum (ER) and other organelle membranes.
Jen Nguyen et al.
Nature communications, 12(1), 3662-3662 (2021-06-18)
It has long been known that bacteria coordinate their physiology with their nutrient environment, yet our current understanding offers little intuition for how bacteria respond to the second-to-minute scale fluctuations in nutrient concentration characteristic of many microbial habitats. To investigate the effects
W Colomb et al.
Journal of microscopy, 266(3), 298-306 (2017-03-23)
Fiducial markers are used to correct the microscope drift and should be photostable, be usable at multiple wavelengths and be compatible for multimodal imaging. Fiducial markers such as beads, gold nanoparticles, microfabricated patterns and organic fluorophores lack one or more
Karl Zhanghao et al.
Nature communications, 11(1), 5890-5890 (2020-11-20)
Lipid membranes are found in most intracellular organelles, and their heterogeneities play an essential role in regulating the organelles' biochemical functionalities. Here we report a Spectrum and Polarization Optical Tomography (SPOT) technique to study the subcellular lipidomics in live cells.
Paul T Toran et al.
Experimental hematology, 86, 28-42 (2020-05-31)
As important vectors for ectopic protein expression, gene silencing, and progenitor cell barcoding, lentiviruses continue to emerge as versatile research and clinical tools. For studies employing cell types that are relatively resistant to transduction, high-titer lentivirus preparations with low cytotoxicity
Justin V Joseph et al.
PloS one, 10(12), e0145393-e0145393 (2015-12-25)
Glioblastoma (GBM) is a highly infiltrative brain tumor in which cells with properties of stem cells, called glioblastoma stem cells (GSCs), have been identified. In general, the dominant view is that GSCs are responsible for the initiation, progression, invasion and
Ildikó Kristó et al.
Biochimica et biophysica acta. Molecular cell research, 1864(10), 1589-1604 (2017-05-31)
Current models imply that the evolutionarily conserved, actin-binding Ezrin-Radixin-Moesin (ERM) proteins perform their activities at the plasma membrane by anchoring membrane proteins to the cortical actin network. Here we show that beside its cytoplasmic functions, the single ERM protein of
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